Table 2

AS-PCR test versus Sanger for evaluable specimens and versus Sanger sequencing with MPP to resolve discordant results (n=113)

Mutation detectedMutation not detected
Sanger sequencing
AS-PCR test
 Mutation detected836*
 Mutation not detected1†23
Sanger sequencing with MPP resolution
AS-PCR test
 Mutation detected890
 Mutation not detected024

  • *Two exon 19 deletions; four L858R mutations.

  • †Exon 20 insertion.

  • Sanger Sequencing

  • Positive percent agreement=98.8% (95% CI 94.8 to 99.7).

  • Negative percent agreement=79.3% (95% CI 64.7 to 88.9).

  • Overall percent agreement=89.8% (95% CI 89.0 to 96.6).

  • Sanger sequencing with MPP resolution

  • Positive percent agreement=100% (95% CI 97.0 to 100).

  • Negative percent agreement=100% (95% CI 89.9 to 100).

  • Overall percent agreement=100% (95% CI 97.7 to 100).

  • AS-PCR test, allele-specific PCR; MPP, massively parallel pyrosequencing.