Mutation detected | Mutation not detected | |
---|---|---|
Sanger sequencing | ||
AS-PCR test | ||
Mutation detected | 83 | 6* |
Mutation not detected | 1† | 23 |
Sanger sequencing with MPP resolution | ||
AS-PCR test | ||
Mutation detected | 89 | 0 |
Mutation not detected | 0 | 24 |
*Two exon 19 deletions; four L858R mutations.
†Exon 20 insertion.
Sanger Sequencing
Positive percent agreement=98.8% (95% CI 94.8 to 99.7).
Negative percent agreement=79.3% (95% CI 64.7 to 88.9).
Overall percent agreement=89.8% (95% CI 89.0 to 96.6).
Sanger sequencing with MPP resolution
Positive percent agreement=100% (95% CI 97.0 to 100).
Negative percent agreement=100% (95% CI 89.9 to 100).
Overall percent agreement=100% (95% CI 97.7 to 100).
AS-PCR test, allele-specific PCR; MPP, massively parallel pyrosequencing.