Results of Her2, β-actin and tubulin SRM assays performed in triplicate are shown
| Her2 | Actin | Tubulin | xScisor (µM) | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Section | Area dissected (mm2) | Protein recovered (µg) | SRM (amol/µg) | CV (%) | SRM (fmol/µg) | CV (%) | SRM (fmol/µg) | CV (%) | |
| 15 | 12 | 11.13 | 517.41 | 8.68 | 514.52 | 2.47 | 110.78 | 3.49 | 200 |
| 16 | 8 | 6.4 | 424.25 | 8.28 | 508.67 | 1.98 | 107.75 | 2.14 | 200 |
| 17 | 8 | 5.6 | 453.86 | 8.35 | 525.49 | 2.35 | 120.35 | 2.84 | 200 |
| 18 | 8 | 9.6 | 536.46 | 7.97 | 486.54 | 3.25 | 106.35 | 3.48 | 200 |
| Laser—12 | 8 | 6.7 | 597.5 | 2.26 | 588.83 | 1.57 | 127.65 | 3.22 | NA |
| Scalpel—11 | NA | 8.2 | 168.70 | 3.06 | 279.15 | 0.14 | 57.45 | 6.57 | NA |
Total area dissected and amount of protein obtained from that area is shown along with quantitative proteomic data (including CV) for the manual, laser-based and mesodissected serial sections.
SRM, selected reation monitoring.