Effect of time of fixation on Ki-67 immunohistochemistry
| Conditions | No. of samples | Ki-67 index (mean±SD: %) | p Value (paired t test) |
|---|---|---|---|
| Time to fixation | |||
| Immediate | 10 | 43.1±11.5 | 0.0284 |
| 16 h | 36.7±18.4 | ||
| Insufficient fixation | |||
| 48 h | 8 | 21.8±10.8 | 0.0177 |
| 3 h | 8.8±9.4 | ||
| Prolonged fixation | |||
| 48 h | 10 | 36.3±13.6 | vs 48 h |
| 14 days | 26.6±15.8 | 0.0414 | |
| 28 days | 22.4±11.2 | 0.0042 | |
| 56 days | 14.4±6.5 | 0.0003 | |