Table 3

Mutation concordance analysis for the Idylla NRAS-BRAF mutation test for NRAS compared with MassARRAY. Table entries on the diagonal with orange shading are concordant results. The one discordant result, G12D with Idylla and WT with MassARRAY, is shaded light blue and discussed in the text.

Idylla	MassARRAY
Idylla	G12A	G12V	G12C	G12D	G12S	G13D	Q61H	Q61K	Q61L	G61R	A146T/V	WT
G12A/V	1	4	0	0	0	0	0	0	0	0	0	0
G12C	0	0	2	0	0	0	0	0	0	0	0	0
G12D	0	0	0	11	0	0	0	0	0	0	0	1*
G12S	0	0	0	0	0	0	0	0	0	0	0	0
G13D	0	0	0	0	0	1	0	0	0	0	0	0
Q61H	0	0	0	0	0	0	1	0	0	0	0	0
Q61K	0	0	0	0	0	0	0	7	0	0	0	0
Q61L	0	0	0	0	0	0	0	0	3	0	0	0
G61R	0	0	0	0	0	0	0	0	0	6	0	0
A146T/V	0	0	0	0	0	0	0	0	0	0	0	0
WT	0	0	0	0	0	0	0	0	0	0		205

The orange entries on the diagonal highlight the correspondence between the MassARRAY and Idylla results. The one discrepant result has a light blue background.
*ddPCR confirmed the G12D result (<5% allelic frequency).
WT, wild type.