Primers used for drug-resistant gene amplification
| Genes | Primers (sequences 5'→3') | Product size (bp) |
| rpoB | F (5′-ACGGTCGGCGAGCTGATCC-3′) R (5′-CAGACCGATGTTGGGCCCCT-3′) | 351 |
| katG | F (5′-GACATTCGCGAGACGTTTCGG-3′) R (5′-GCTCTTAAGGCTGGCAATCTCG-3′) | 469 |
| inhA | F (5′-CTATATCTCCGGTGCGGTCA-3′) R (5′-CTTGGCCATCGAAGCATAC-3′) | 469 |
| rpsL | F (5′-AGGTCACGGCGTACATTCC-3′) R(5′-GCCCTTCTCCTTCTTAGCG-3′) | 188 |
| embB | F (5′-TGATATTCGGCTTCCTGCTC-3′) R (5′-ACCGCTCGATCAGCACATAG-3′) | 417 |
embB, EMB mutant gene; katG and inhA, INH mutant gene; rpoB, RFP mutant gene; rpsL, SM mutant gene.