Regular ArticleSynergistic Effects of IL-6 and IFN-γ on Carcinoembryonic Antigen (CEA) and HLA Expression by Human Colorectal Carcinoma Cells: Role for Endogenous IFN-β
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Interferon regulatory factor 1 and a variant of heterogeneous nuclear ribonucleoprotein L coordinately silence the gene for adhesion protein CEACAM1
2018, Journal of Biological ChemistryCitation Excerpt :Colon carcinoma cells transfected with DCC also exhibited suppressed proliferation and down-regulation of CEACAM5 expression (36). To validate that DCC is down-regulated because of the interaction between Lv1-F and IRF1, we chose MCF7 breast cancer cells that express DCC and CEACAM5 under the regulation of IFN-γ (37), but have undetectable levels of CEACAM1 (38). When the expression levels of DCC were determined by Western blotting with or without Lv1-F in the presence IRF1, we observed more than 58% down-regulation of DCC (Fig. 6, D (lane 4 versus 3) and E (100–41.6)) in agreement with our ChIP-Seq dataset.
Neutralization of interleukin (IL)-10 released by monocytes/macrophages enhances the up-regulatory effect of monocyte/macrophage-derived IL-6 on expressions of IL-6 and MUC1, and migration in HT-29 colon cancer cells
2010, Cellular ImmunologyCitation Excerpt :Blocking of IL-6 and IL-6 receptors using monoclonal antibodies effectively prevented IL-6/tyrosine phosphorylation (Tyr705) of STAT3 proteins in Colo 205 cells [1]. Further, IL-6 stimulated clonogenic growth of colon cancer cell lines and induced expression of surface antigens such as carcinoembryonic antigen (CEA) and HLA-I [24,25]. These findings suggest that IL-6 may modulate the biological behavior of colon cancer and allow cells to disseminate and survive at distant sites.
Interleukin-6 (IL-6) released by macrophages induces IL-6 secretion in the human colon cancer HT-29 cell line
2009, Human ImmunologyCitation Excerpt :Among colon cancers, IL-6 levels are correlated with tumor stage, survival rate, and liver metastasis [19–23]. In in-vitro studies, IL-6 stimulated clonogenic growth of colon cancer cell lines and induced expression of surface antigens such as carcinoembryonic antigen (CEA) and human leukocyte antigen (HLA)-I [24,25]. The addition of exogenous IL-6 to the Colo 205 colon cancer line stimulated STAT3 phosphorylation and subsequently drove interleukin-10 (IL-10) secretion.
Macrophage-derived interleukin-6 up-regulates MUC1, but down-regulates MUC2 expression in the human colon cancer HT-29 cell line
2009, Cellular ImmunologyCitation Excerpt :Studies regarding cancers of the prostate, ovary, and head and neck have further demonstrated that this activation of the IL-6/STAT3 signaling pathway is associated with tumor growth [4–6]. In in vitro studies, IL-6 is capable of stimulating clonogenic growth of colon cancer cell lines and inducing expression of surface antigens, such as carcinoembryonic antigen (CEA), and HLA-I molecules [7,8]. Addition of exogenous IL-6 to a colon cancer line, Colo 205, stimulates STAT3 phosphorylation and subsequently drives interleukin-10 (IL-10) secretion by cancer cells.
Combined effects of protein kinase inhibitors and 5-fluorouracil on CEA expression in human colon cancer cells
2005, Pharmacological Research