Regular Article
Anin VitroModel to Assess Mucosal Immune Function and Bacterial Translocation

https://doi.org/10.1006/jsre.1997.5070Get rights and content

Abstract

The importance of secretory immunoglobulin A (IgA) on intestinal barrier function has gained increasing acceptance. However, due to the complexity of the intestinal microenvironment, the relative role of secretory IgA (sIgA) in mucosal defense has been difficult to studyin vivo.Polarized Madin–;Darby canine kidney (MDCK) epithelial cells expressing the complementary DNA (cDNA) for the polymeric Ig receptor were grown as monolayers in anin vitrotwo-chamber cell culture system to study the impact of sIgA on bacterial translocation (BT). Polymeric sIgA or media alone was added to the apical chambers of cell monolayers followed by apical inoculation with bacteria. The basal compartment was sampled at timed intervals thereafter to determine BT. Bacterial passage across the MDCK epithelial cell monolayers occurred in a time and bacterial inoculum concentration gradient. Addition of sIgA led to significant reductions in BT across the epithelial cell monolayers. This is a useful model for further investigation on the role of sIgA in intestinal barrier function.

References (19)

  • K.E. Mostov et al.

    Polymeric immunoglobulin receptor expressed in MDCK cells transcytoses IgA

    Cell

    (1986)
  • E.A. Deitch

    The role of intestinal barrier failure and bacterial translocation in the development of systemic infection and multiple organ failure

    Arch. Surg.

    (1990)
  • W.F. Doe

    The intestinal immune system

    Gut

    (1989)
  • B. LangKamp-Henken et al.

    Immunological structure and function of the gastrointestinal tract

    Nutr. Clin. Practice

    (1992)
  • R.C. Williams et al.

    Inhibition of bacterial adherence by secretory immunoglobin: A mechanism of antigen disposal

    Science

    (1972)
  • M.B. Mazenec et al.

    Intracellular neuralization of virus by immunoglobin A antibodies

    Proc. Natl. Acad. Sci. USA

    (1992)
  • C.S. Kaetzel et al.

    The polymeric immunoglobin receptor (secretory component) mediates transport of immune complexes across epithelial cells: A local defense function for IgA

    Proc. Natl. Acad. Sci. USA

    (1991)
  • N. Kruz et al.

    Bacterial translocation is bacterial species dependent: Results using the human CaCO2 intestinal cell line

    J. Trauma

    (1994)
  • J.W. Alexander et al.

    The process of microbial translocation

    Ann. Surg.

    (1990)
There are more references available in the full text version of this article.

Cited by (8)

  • Breaching host defenses in the urinary tract

    2002, Hong Kong Journal of Nephrology
  • Synergistic effects of Candida and Escherichia coil on gut barrier function

    1999, Journal of Trauma - Injury, Infection and Critical Care
View all citing articles on Scopus

K. S. MatlinJ. D. Valentich, Eds.

View full text