Regular paperUse of the polymerase chain reaction to specifically amplify integrated HPV-16 DNA by virtue of its linkage to interspersed repetitive DNA
References (0)
Cited by (20)
Understanding the HPV integration and its progression to cervical cancer
2018, Infection, Genetics and EvolutionCitation Excerpt :The first techniques used for the detection of HPV in cells were immunohistochemistry and Fluorescent in situ hybridization (FISH), using monoclonal antibodies targeting cell proteins such as p16, which is produced in the presence of HPV infection, primarily when E7 oncogene is expressed (Park et al., 1999a; Sano et al., 1998; Rufforny et al., 2005), and probes for HPV sequences allowing the fluorescent detection of small segments of the viral genome in the chromosomes of the host cell (Lizard et al., 1994; Siadat-Pajouh et al., 1994; Adler et al., 1997). Later, HPV detection was performed by polymerase chain reaction (PCR) (Carmody et al., 1996), and it was possible to detect the presence of the E2, E6, and E7 genes, allowing researchers to determine the viral state in the infected cell (episomal/integrated) by measuring the amplification of these sequences (Donaldson et al., 1993). These integration-detecting methods improved with real-time PCR, with the ability to observe with higher precision the E2/E6 or E2/E7 ratio in real time (Zheng et al., 2006; Fujii et al., 2005).
A repetitive DNA sequence that characterizes human papillomavirus integration site into the human genome is present in vulvar vestibulitis
2000, European Journal of Obstetrics and Gynecology and Reproductive BiologyWhole Genome Assembly of Human Papillomavirus by Nanopore Long-Read Sequencing
2022, Frontiers in Genetics
- f1
Author to whom correspondence should be addressed.