Abstract
Firstly, to determine a satisfactory animal model for induction of intrarenal calcification, a study of four previously described animal models of intrarenal calcification was carried out which showed that intraperitoneal injection of 10% calcium gluconate into female Sprague-Dawley rats was most effective. We then investigated the hypothesis that dietary supplementation with essential fatty acids could reduce the intrarenal calcification developing as a result of intraperitoneal calcium injection. Using a combination of fish oil and evening primrose, oil, we demonstrated a significant difference in renal parenchymal calcification, which was 940±240 μg Ca/g dry weight renal parenchyma in unsupplemented animals and 320–370 ±55–65 μg Ca/g dry weight renal parenchyma in supplemented animals (means±SEM, P<0.005). It was also demonstrated that there was synergism between eicosapentaenoic acid (EPA) and γ-linolenic acid (GLA): dietary supplementation with a combined oil preparation containing 27 mg/ml EPA and 67 mg/ml GLA mixed as 2% with food was as effective as oils containing either 400 mg/ml EPA or 80 mg/ml GLA mixed as 4% of food.
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Burgess, N.A., Reynolds, T.M., Williams, N. et al. Evaluation of four animal models of intrarenal calcium deposition and assessment of the influence of dietary supplementation with essential fatty acids on calcification. Urol. Res. 23, 239–242 (1995). https://doi.org/10.1007/BF00393305
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DOI: https://doi.org/10.1007/BF00393305