Summary
Types 1 and 2C fibers in human skeletal muscle were cross-reactively identified with monoclonal anti-bovine neurofilament (200 kd) antibody. Thirty seven biopsy samples including sixteen vastus lateralis muscles, twelve lumbar paravertebral muscles, six gluteus medius muscles, two flexor carpi ulnaris muscles, and one flexor pollicis longus muscle, were examined. Serial transverse sections were stained histochemically with myofibrillar ATPase (pH 10.4, 4.6, 4.3) and DPNH-tetrazolium reductase reactions, and immunochemically using the avidin-biotin-peroxidase complex with the primary antibodies of monoclonal anti-bovine neurofilament (200 kd, 160 kd, 70 kd) antibodies and anti-bovine glial filament acidic protein antibody. The immunochemical reaction with anti-NF (200 kd) antibody could distinguish two kinds of fibers; positive and negative in all of the specimens. No fiber was recognized with other antibodies. Myosin ATPase reactions in serial sections proved that the positively stained fibers with anti-NF (200 kd) antibody were types 1 and 2C fibers and negative fibers types 2A and 2B fibers. At present, it is not known what substance is responsible for the cross-reaction with the monoclonal anti-NF (200 kd) antibody in types 1 and 2C fibers, but this unique antibody would be valuable in two aspects: one concerns the problem of the evolution of fiber types, and the other the utility as another supplemental method to conventional myosin ATPase scheme.
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References
Arndt I, Pepe FA (1975) Antigenic specificity of red and white muscle myosin. J Histochem Cytochem 23:159–168
Bennett GS, Tapscott SJ, DiLullo C, Holtzer H (1984) Differential binding of antibodies against the neurofilament triplet proteins in different avian neurons. Brain Res 304:291–302
Billeter R, Weber H, Lutz H, Howald H, Eppenberger HM, Jenny E (1980) Myosin types in human skeletal muscle fibers. Histochemistry 65:249–259
Brooke MH, Kaiser KK (1970a) Three “myosin ATPase” systems: The nature of their pH lability and sulfhydryl dependence. J Histochem Cytochem 18:670–672
Brooke MH, Kaiser KK (1970b) Muscle fiber types: How many what kind? Arch Neurol 23:369–379
Brown BA, Majocha RE, Staton DM, Marotta CA (1981a) Axonal polypeptides cross-reactive with antibodies to neurofilament protein. J Neurochem 36:143–153
Brown BA, Nixon RA, Strocchi PS, Marotta CA (1981b) Characterization and comparison of neurofilament proteins from rat and mouse CNS. J Neurochem 36:143–153
Carpenter S, Karpati G (1984) Pathology of skeletal muscle, Churchill Livingstone, New York, p 225
Dhoot GK, Perry SW (1979) Distribution of polymorphic forms of troponin components and tropomyosin in skeletal muscle. Nature 278:714–718
Dhoot GK, Gell PGH, Perry SV (1978) The localization of the different forms of troponin I in skeletal and cardiac muscle cells. Exp Cell Res 117:357–370
Dubowitz V, Brooke MH (1973) Muscle biopsy: A modern approach. WB Saunders, London, pp 51, 98–102
Garnett RAF, O'Donovan MJ, Stephens JA, Taylor A (1978) Motor unit organization of human medial gastrocnemius. J Physiol 287:33–43, 1978
Gauthier GF, Lowey S (1977) Polymorphism of myosin among skeletal muscle fiber types. J Cell Biol 74:760–779
Gauthier GF, Lowey S, Hobbs AW (1978) Fast and slow myosin in developing muscle fibers. Nature 274:25–29
Gröschel-Stewart U, Meschede D, Lehr I (1973) Histochemical and immunochemical studies on mammalian striated muscle fibers. Histochemie 33:79–85
Guth L, Samaha FJ (1969) Qualitative differences between actomyosin ATPase of slow and fast mammalian muscle. Exp Neurol 25:138–152
Hirokawa N, Glicksman MA, Willand M (1982) Organization of mammalian neurofilamet polypeptides within axonal cytoskeleton. J Cell Biol 95:236a
Hsu SM, Rame L, Panger A (1981) A comparative study of the peroxidase-antiperoxidase method and an avidin-biotin complex method for studying polypeptide hormones with radioimmunoassay antibodies. Am J Clin Pathol 75:734–738
Jansson E, Kaijser L (1977) Muscle adaptation to extreme endurance training in man. Acta Physiol Scand 100:315–324
Jansson E, Sjodin B, Taesch P (1978) Changes in muscle fiber type distribution in man after physical training. A sign of fiber type transformation? Acta Physiol Scand 104:235–237
Jennekens FGJ, Tomlinson BE, Walton JN (1971) Histochemical aspects of five limb muscles in old age. An autopsy study. J Neurol Sci 14:259–276
Labsystems (1985) Labsystems mouse monoclonal antibodies to neurofilaments. Information distributed by Labsystem, Helsinki, Finland
Lee V, Wu HL, Schlaepfer WW (1982) Monoclonal antibodies recognize individual neurofilament triplet protein. Proc Natl Acad Sci USA 79:6089–6092
Liem RKH, Yen SH, Salomon GD, Schelanski ML (1978) Intermediate filaments in nervous tissue. J Cell Biol 79:637–645
Lutz H, Ermini M, Jenny E, Bruggman S, Joris F, Weber E (1978) The size of the fiber populations in rabbit skeletal muscles as revealed by indirect immunofluorescence with anti-myosin sera. Histochemistry 57:223–235
Lutz H, Weber H, Billeter R, Jenny E (1979) Fast and slow myosin within single skeletal muscle fibers of adult rabbits. Nature 281:142–144
Padykula HA, Herman E (1955) The specificity of the histochemical method of adenosine triphosphate. J Histochem Cytochem 3:170–183
Rowlerson A (1978) Fluorescent-labelled antibody to fast and slow muscle myosin in the study of mammalian muscle fiber specialization. J Physiol (Lond) 282:29–30
Rubinstein N, Mabuchi K, Pepe F, Salmons S, Gergely J, Streter F (1978) Use of type specific antimyosins to demonstrate the transformation of individual fibers in chronically stimulated rabbit fast muscles. J Cell Biol 79:252–261
Schantz P, Billeter R, Henriksson J, Jansson E (1982) Training-induced increase in myofibrillar ATPase intermediate fibers in human skeletal muscle. Muscle Nerve 5:628–636
Schlaepfer WW, Lee V, Wu HL (1981) Assessment of immunological properties of neurofilament triplet protein. Brain Res 226:259–272
Sharp GA, Shaw G, Weber K (1982) Immunoelectron microscopic localization of three neurofilament triplet proteins along neurofilaments of cultured dorsal root ganglion neurons. Exp Cell Res 137:403–413
Shaw G, Osborn M, Weber K (1981) An immunofluorescence microscopical study of the neurofilament triplet proteins, vimentin and glial fibrillary acidic protein within the adult rat brain. Eur J Cell Biol 26:68–82
Staron RS, Hikida RS, Hagerman C, Dudley GA, Murray TF (1984) Human skeletal muscle fiber type adaptability to various workloads. J Histochem Cytochem 32:146–152
Taylor CB (1978) Immunoperoxidase technics, practical and theoretical aspects. Arch Pathol Lab Med 102:113–121
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Nakamura, T., Kawahara, H., Miyashita, H. et al. Cross reactive identification of types 1 and 2C fibers in human skeletal muscles with monoclonal anti-neurofilament (200 kd) antibody. Histochemistry 87, 39–45 (1987). https://doi.org/10.1007/BF00518722
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DOI: https://doi.org/10.1007/BF00518722