Abstract
Proliferating cell nuclear antigen, PCNA (PC10), immunolabelling was determined in 175 women with breast carcinomas and related to other established prognostic factors: flow-cytometric data, volume-corrected mitotic index, sex steroid receptor content and clinical outcome during the mean follow-up of 9 years. The maximum fraction of PCNA-positive nuclei (PCNAmax), the average fraction of positive nuclei (PCNAtot) and the number of intensely stained nuclei per microscope field (PCNAcount) were significantly related to histological grade (P<0.001), DNA ploidy ((P<0.001), S-phase fraction (P<0.001), mitotic index (P<0.001) and sex steroid receptor content (P=0.002). PCNAmax (P=0.015) predicted survival in univariate analysis; PCNAtot (P=0.025), PCNAmax (P=0.007) and PCNAcount (P=0.019) predicted the recurrence-free survival. In axillary-lymph-node-negative tumours, PCNAtot (P=0.092), PCNAmax (P=0.036) and PCNAcount (P=0.006) predicted survival and recurrence-free survival (P=0.011), (P=0.012) and (P=0.006) respectively. In multivariate analysis including clinical, histological, flow-cytometric and biochemical variables, PCNAtot (P=0.004) predicted the recurrence-free survival independently. In axillary-lymph-node-negative breast cancers, PCNAtot predicted accurately the patient survival (P=0.002) and the recurrence-free survival (P=0.002). The results indicate that PCNA immunolabelling has independent prognostic value particularly in local breast cancer.
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Abbreviations
- PCNA:
-
proliferating-cell nuclear antigen
References
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Aaltomaa, S., Lipponen, P., Papinaho, S. et al. Proliferating-cell nuclear antigen (PC10) immunolabelling and other proliferation indices as prognostic factors in breast cancer. J Cancer Res Clin Oncol 119, 288–294 (1993). https://doi.org/10.1007/BF01212727
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DOI: https://doi.org/10.1007/BF01212727