Skip to main content
SpringerLink
Log in

Immunocytochemical study of progesterone receptor in human meningioma

  • Research Articles
  • Published:
Acta Neurochirurgica Aims and scope Submit manuscript

Summary

Progesterone (PR) and oestrogen (ER) receptors were examined in meningiomas from 36 patients, using immunocytochemistry. The present experiments were performed to evaluate: (a) the presence and intracellular localization of these receptors, (b) whether PR immunostaining can be correlated (or not) with proliferation potential, as evaluated by histopathological features or the clinical evolution of this neuropathological tumour.

Twenty six tumours (72%) tested were positive for PR but none for ER. The presence of PR immunostaining was more frequently observed in females (79% versus 58% in males) and premenopausal status (84% versus 3/5 in postmenopausal). Correlations of PR immunostaining with the histologic type showed 89% of meningothelial, 4/6 cases of transitional, 1/3 case of fibroblastic and 1/4 cases of anaplastic meningiomas to be immunostained for PR. Staining was confined to tumours arachnoidal cells. A heterogeneous distribution was observed in most PR-positive meningiomas. The preferential immunostaining in meningothelial histological types correlates with the presence of PR in normal arachnoidal cells. The proliferating potential of these meningiomas was evaluated by the immunostaining of an antigen only present in proliferating cells (Ki antigen). There was no significant correlation between PR status and the Ki labelling rate, or rapid clinical evolution.

These data were compared with those previously reported. They confirm that the cellular biosynthesis of PR in meningiomas is not oestrogen regulated as it is in other sex steroid tissues, such as the breast and the endometrium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bayard F, Damilano S, Robel P, Baulieu EE (1978) Cytoplasmic and nuclear estradiol and progesterone receptors in human endometrium. J Clin Endocrinol Metab 46: 635–648

    PubMed  Google Scholar 

  2. Bergeron Ch, Ferenczy A, Shyamala G (1988) Distribution of oestrogen receptors in various cell types of normal, hyperplastic, and neoplastic human endometrial tissues. Lab Invest 58, 3: 338–345

    Google Scholar 

  3. Bickerstaff ER, Small JM, Guest IA (1958) The relapsing course of certain meningioma in relation to pregnancy and menstruation. J Neurol Neurosurg Psychiatry 21: 89–91

    Google Scholar 

  4. Blankenstein MA, Berns PMJJ, Blaauw G, Mulder E, Thijssen JHH (1986) Search for oestrogen receptors in human meningioma tissue sections with a monoclonal antibody against the human oestrogen receptor. Cancer Res [Suppl] 46:4268s-4270s

    Google Scholar 

  5. Blankenstein MA, Blaauw G, Lamberts SW, Mulder E (1983) Presence of progesterone receptors and absence oestrogen receptors in human intracranial meningioma cytosols. Eur J Cancer Clin Oncol 19: 365–370

    PubMed  Google Scholar 

  6. Blankenstein MA, Van der Meulen-Dijk C, Weigand RA, Thijssen JHH (1988) Progestin and oestrogen receptor assay in human meningioma cytosols by enzyme immunoassay In: Bresciani F, King RJB, Lipman ME, Raynaud JP (eds) Progr in Cancer Research and Therapy, Vol 35. Raven Press, New York, pp 71–74

    Google Scholar 

  7. Blankenstein MA, Van't Verlaat JW, Croughs RJM (1989) Hormone dependency of meningiomas. The Lancet 8651: 1381–1382

    Google Scholar 

  8. Blaustein JD, King JC, Toft DO, Turcotte J (1988) Immunocytochemical localization of oestrogen-induced progestin receptors in guinea pig brain. Brain Res 474: 1–15

    PubMed  Google Scholar 

  9. Donnell MS, Glenn BA, Meyer A, Donegan Wl (1979) Oestrogen-receptor protein in intracranial meningiomas. J Neurosurg 50: 499–502

    PubMed  Google Scholar 

  10. Garcia E, Bouchard P, De Brux J, Berdah J, Frydman R, Schaison G, Milgrom E, Perrot-Applanat M (1988) Use of immunocytochemistry of progesterone and oestrogen receptors for endometrial dating. J Clin Endocrinol Metab 67: 80–87

    PubMed  Google Scholar 

  11. Gerdes J, Lemke H, Baisch Het al. (1984) Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki 67. J Immunol 133: 1710–1715

    PubMed  Google Scholar 

  12. Gerdes J, Schwab U, Lemke H, Stein H (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer 31: 13–20

    PubMed  Google Scholar 

  13. Greene GL, Harris K, Bova R, Kinders R, Moore B, Nolan C (1988) Purification of T 47 D human progesterone receptor and immunochemical characterization with monoclonal antibodies. Mol Endocrinol 2: 714–726

    PubMed  Google Scholar 

  14. Greene GL, Nolan C, Engler J, Jensen EV (1980) Monoclonal antibodies to human oestrogen receptor. Proc Natl Acad Sci U.S.A. 77: 5115–5119

    PubMed  Google Scholar 

  15. Jung-Testas I, Renoir JM, Gasc JM Baulieu EE (1991) Estrogeninducible progesterone receptor in primary cultures of rat glial cells. Exp Cell Res 193: 12–19

    PubMed  Google Scholar 

  16. Katznellenbogen BS (1980) Dynamic of steroid hormone receptor action. Annu Rev Physiol 42: 17

    PubMed  Google Scholar 

  17. Kepes JJ (1982) Meningiomas biology, pathology and differential diagnosis, Vol 1. Masson Publishing U.S.A. INC, New York, pp 206

    Google Scholar 

  18. King WJ, DeSombre ER, V Jensen E, Green GL (1985) Comparison of immuno-cytochemical and steroid-binding assays for oestrogen receptor in human breast tumors. Cancer Res 45: 293–304

    PubMed  Google Scholar 

  19. Lessey BA, Killam AP, Metzger DA, Haney AF, Greene GL, McCarty KS Ir (1988) Immunohistochemical analysis of human uterine oestrogen and progesterone receptors throughout the menstrual cycle. J Clin Endocrinol Metab 67: 334–340

    PubMed  Google Scholar 

  20. Logeat F, Pamphile R, Loosfelt R, Jolivet H, Fournier A, Milgrom E (1985) Onestep immunoafinity purification of active progesterone recpetor. Further evidence in favour of the existence of a single steroid binding subunit. Biochemistry 24: 1029–1035

    PubMed  Google Scholar 

  21. Logeat F, Vu Hai MT, Fournier A, Legrain P, Butin G, Milgrom E (1983) Monoclonal antibodies to rabbit progesterone receptor: crossreaction with other mammalian progesterone receptors. Proc Natl Acad Sci U.S.A. 80: 6456–6459

    PubMed  Google Scholar 

  22. Lorenzo F, Jolivet A, Loosfelt H, Vu Hai MT, Brailly S, PerrotApplanat M, Milgrom E (1988) A rapid method of epitopemapping. Application to the study of immunogenic domains and to the characterization of various forms of rabbit progesterone receptor. Eur J Biochem 176: 53–56

    PubMed  Google Scholar 

  23. MacLusky NJ, McEven BS (1978) Oestrogen modulates progestin receptor concentrations in some rat brain regions but not in others. Nature 274: 276–278

    PubMed  Google Scholar 

  24. Markwalder TM, Gerber HA, Waelti E, Schaffner T, Markwalder R (1988) Hormonotherapy of meningiomas with medroxyprogesterone acetate. Immunohistochemical demonstration of the effect of medroxyprogesterone acetate on growth fractions of meningioma cells using the monoclonal antibody Ki 67. Surg Neurol 30: 97–101

    PubMed  Google Scholar 

  25. Markwalder TM, Zava DT, Goldhirsch A, Markwalder RV (1983) Estrogen and progesterone receptors in meningiomas in relation to clinical and pathologic features. Surg Neurol 20: 42–47

    PubMed  Google Scholar 

  26. Olson JJ, Beck DW, Schlechte J, Loh PM (1986) Hormonal manipulation of meningiomas in vitro. J Neurosurg 65: 99–107

    PubMed  Google Scholar 

  27. Olson JJ, Beck DW, Schlechte JA, Loh PM (1987) Effect of the antiprogesterone RU-38486 on meningioma implanted into nude mice. J Neurosurg 66: 584–587

    PubMed  Google Scholar 

  28. Perrot-Applanat M, Groyer-Picard MT, Lorenzo F, Jolivet A, Vu Hai MT, Pallud C, Spyratos F, Milgrom E (1987) Immunocytochemical study with monoclonal antibodies to progesterone receptor in human breast tumors. Cancer Res 47: 2652–2661

    PubMed  Google Scholar 

  29. Perrot-Applanat M, Groyer-Picard MT, Vu Hai MT, Pallud C, Spyratos F, Milgrom E (1989) Immunocytochemical staining of progesterone receptor in paraffin sections of human breast cancers. Am J Path 135: 457–468

    PubMed  Google Scholar 

  30. Pertuiset BF, Moguilewsky M, Magdalenat H, Martin PM, Philibert D, Poisson M (1984) Sex steroid receptors in human meningioma and glioma. In: Bresciani F (ed) Progr. in Cancer Research and Therapy, vol.31. Raven Press, New York, pp 561–568

    Google Scholar 

  31. Ploton D, Menager M, Jeannesson Pet al (1986) Improvement in the staining and in the visualization of argyrophilic proteins of the nucleolar organizer region at the optical level. Histochem J 58: 5–14

    Google Scholar 

  32. Poisson M, Magdalenat H, Foncin JF, Bleibel JM, Philippon J, Pertuiset B, Buge A (1980) Recepteurs d'oestrogène et de progestérone dans les Méningiomes. Rev Neurol (Paris) 136: 193–203

    Google Scholar 

  33. Schnegg JF, Gomez F, Lemarchand-Beraud T, De Tribolet N (1981) Presence of sex steroid hormone receptors in meningioma tissue. Surg Neurol 15: 415–418

    PubMed  Google Scholar 

  34. Schoenberg BS, Christine BW, Whisnant JP (1975) Nervous system neoplasms and primary malignancies of other sites: the unique association between meningiomas and breast cancer. Neurology 25: 705–712

    PubMed  Google Scholar 

  35. Schott W, Buchfelder M, Schrell U, Fahlbusch R (1987) Sexual steroid receptors in meningiomas. Acta Endocrinol [Suppl] 114: 136–137

    Google Scholar 

  36. Schrell UMH, Adams EF, Fahlbusch R (1989) Hormone dependency of meningiomas. Lancet 8651: 1381

    Google Scholar 

  37. Simpson D (1957) The recurrence of intracranial meningiomas. J Neurol Neurosurg Psychiatry 20: 22–39

    Google Scholar 

  38. Sprangers SA, West NB, Brenner RM, Bethea CL (1990) Regulation and localization of estrogen and progestin receptors in the pituitary of steroid-treated monkeys. Endocrinology 126: 1133–1142

    PubMed  Google Scholar 

  39. Vu Hai MT, Jolivet A, Ravet V, Lorenzo F, Perrot-Applanat M, Citerne M, Milgrom E (1989) Novel monoclonal antibodies against human uterine progesterone receptor. Mapping of receptor immunogenic domains. Biochem J 260: 371–376

    PubMed  Google Scholar 

  40. Warembourg M, Jolivet A, Milgrom E (1989) Immunohistochemical evidence of the presence of estrogene and progesterone receptors in the same neurons of the guinea pig hypothalamus and preoptic area. Brain Res 480: 1–15

    PubMed  Google Scholar 

  41. Weyand RD, MacCarty CS, Wilson RB (1951) The effect of pregnancy on intracranial meningiomas occuring about the optic chiasm. Surg Clin North Am 31: 1225–1233

    PubMed  Google Scholar 

  42. Yu ZY, Wrange O, Haglund B, Granholm L, Gustafsson JA (1982) Estrogen and progestin receptors in intracranial meningiomas. J Steroid Biochem 16: 451–456

    PubMed  Google Scholar 

  43. Zava DT, Markwalder TM, Markwalder R (1984) Biological expression of steroid hormone receptors in primary meningioma cells in monolayer culture. Clin Neuropharmacol 7: 382–388

    PubMed  Google Scholar 

  44. Zülo KJ (1979) Histological classification of tumors of the central nervous system. International histological classification of tumours n ∘ 21, WHO, Geneva, pp 47–60

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Faculté de Médecine Paris Sud, Groupe de Recherches Hormones et Reproduction (INSERM U 135), Le Kremlin Bicêtre, France

    M. Perrot-Applanat & M. Th. Groyer-Picard

  2. Faculté de Médecine, Laboratoire Histologie-Embryologie et Cytogénétique, Paris, France

    M. Kujas

Authors
  1. M. Perrot-Applanat
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Th. Groyer-Picard
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Kujas
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Supported by the Institut National de la Santé et de la Recherche Médicale; The Centre National de la Recherche Scientifique, The Unité d'Enseignement et de Recherches Kremlin-Bicêtre, The Association pour la Recherche sur le Cancer.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Perrot-Applanat, M., Groyer-Picard, M.T. & Kujas, M. Immunocytochemical study of progesterone receptor in human meningioma. Acta neurochir 115, 20–30 (1992). https://doi.org/10.1007/BF01400586

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01400586

Keywords

Search

Navigation