Summary
This report describes the immunocytochemical localization of a human basal- or myoepithelial-specific antikeratin antibody in benign and malignant breast disease. Reactivity patterns with this antibody have demonstrated the lack of myoepithelial or basal epithelial participation in most benign breast specimens examined including those displaying cystic disease, fibrosis, or hyperplasia. However, in specimens of sclerosing adenosis, strong reactivity with the majority of cells in most ducts suggests a major participation of the myoepithelial cell type. Analysis of 118 breast carcinoma specimens has demonstrated strong, homogeneous reactivity in 4% of the specimens, suggesting a role for the basal epithelial cell in malignancy of the human mammary gland and implications for the prognosis of such tumors. Antigenic characterization of the malignant and benign mammary specimens which are uniformly reactive with the antibody has demonstrated the presence of a 51 kd keratin polypeptide not found in the non-reactive specimens.
References
Linzell JL: Some observations on the contractile tissue of the mammary glands. J Physiol 130: 257–267, 1955
Pinkstaff CA: The cytology of salivary glands. Int Rev Cytol 63: 141–261, 1980
Warburton MJ, Ferns SA, Rudland PS: Enhanced synthesis of basement membrane proteins during the differentiation of rat mammary tumor epithelial cells into myoepithelial cellsin vitro. Exp Cell Res 137: 373–380, 1982
Rudland PS, Gusterson BA, Huges CM, Ormerod EJ, Warburton MJ: Two forms of tumors in nude mice generated by a neoplastic rat mammary stem cell line. Cancer Res 42: 5196–5208, 1982
Carter D, Yardley JH, Shelley WM: Lobular carcinoma of the breast: an ultrastructural comparison with certain duct carcinoma and benign lesions. Johns Hopkins Med J 125: 25–43, 1969
Ozzello L: Epithelial-stromal junction of normal and dysplastic mammary gland. Cancer 25: 586–600, 1970
Ozzello L: Ultrastructure of the human mammary gland. Pathol Ann 6: 1–58, 1971
Ahmed A: The myoepithelium in human breast carcinoma. J Pathol 113: 129–135, 1974
Gould VE, Miller J, Jao W: Ultrastructure of medullary, intraductal, lobular and adenocystic breast carcinomas. Am J Pathol 78: 401–416, 1975
Ohtani H, Sasano N: Myofibroblasts and myoepithelial cells in human breast carcinoma. An ultrastructural study. Virchows Arch A Pathol Anat Histol 385: 247–261, 1980
Gould VE, Jao W, Battifora H: Ultrastructural analysis in the differential diagnosis of breast tumors. Pathol Res Pract 167: 45–70, 1980
Linzell JL: The silver staining of myoepithelial cells, particularly in the mammary gland, and their relation to the ejection of milk. J Anat 86: 49–57, 1952
Van Bogaert LJ, Abarka J, Maldagne P: Appraisal and pitfalls of myoepithelial cells staining by levanol fast cyanine 5RM. Histochem 54: 251–258, 1977
Gabbiani G, Csank-Brassert J, Schneeberger JD, Kapanci Y, Trencher P, Holborow EJ: Contractile proteins in human cancer cells. Am J Pathol 83: 457–474, 1976
Macartney JC, Trevithick MA, Kricka L, Curran RC: Identification of myosin in human epithelial cancers with immunofluorescence. Lab Invest 41: 437–445, 1979
Bussolati G: Actin-rich (myoepithelial) cells in lobular carcinoma in situ of the breast. Virchows Arch B Cell Pathol 32: 165–176, 1980
Bussolati G, Botta G, Gugliotta P: Actin-rich (myoepithelial) cells in ductal carcinoma-in-situ of the breast. Virchows Arch B Cell Pathol 34: 251–259, 1980
Gusterson BA, Warburton MJ, Mitchell D, Ellison M, Munro-Neville A, Rudland PS: Distribution of myoepithelial cells and basement membrane proteins in the normal breast and in benign and malignant breast disease. Cancer Res 42: 4763–4770, 1982
Hyderman E, Steele K, Ormerod MG: A new antigen on the epithelial membrane, its immunoperoxidase localization in normal and neoplastic tissue. J Clin Pathol 32: 35–39, 1979
Taylor-Papadimitriou J, Peterson JA, Arklie J, Burchell J, Ceriani RL, Bodmer WF: Monoclonal antibodies to epithelium-specific components of the human milk fat globule membrane: production and reaction with cells in culture. Int J Cancer 28: 17–21, 1981
Hilkens J, Buijs F, Hilgers J, Hageman PH, Sonnenberg A, Koldovsky U, Karande K, Van Hoeven RP, Feltkamp C, Von de Rign JM: Monoclonal antibodies against human milk fat globule membranes detecting differentiation antigens of the mammary gland. In: Peeters H (ed) Protides of Biological Fluids, Vol 29. Pergamon Press, Oxford 1982, pp. 813–816
Lane EB, Klymkowsky MW: Epithelial tonofilaments: investigating their form and function using monoclonal antibodies. Cold Spring Harbor Symp Quant Biol 46: 387–402, 1981
Dairkee SH, Blayney C, Smith HS, Hackett AJ: Monoclonal antibody that defines human myoepithelium. Proc Natl Acad Sci, USA 82: 7409–7413, 1985
Caselitz J, Walter B, Wustrow J, Seifert G, Weber K, Osborn M: A monoclonal antibody that detects myoepithelial cells in exocrine glands, basal cells in other epithelia, and basal and suprabasal cells in certain hyperplastic tissues. Virchows Arch Pathol Anat 409: 725–738, 1986
Dempsey PJ, DeKretser TA, Brown RW, Whitehead RH, Jose DG: A monoclonal antibody CIBr17 recognizes a myoepithelium-specific antigen in human mammary gland. Int J Cancer 37: 857–866, 1986
Kohler G, Milstein C: Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256: 495–497, 1975
Gown AM, Vogel AM: Monoclonal antibodies to human intermediate filament proteins. Amer J Pathol 114: 309–321, 1984
Moll R, Franke WW, Schiller DL, Geiger B, Krepler R: The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors, and cultured cells. Cell 31: 11–24, 1982
Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 277: 680–685, 1970
Towbin H, Staehlin T, Gordon J: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. Procedure and some applications. Proc Natl Acad Sci, USA 76: 4350–4354, 1979
Robbins SL: Textbook of Pathology with Clinical Applications. WB Saunders, Philadelphia and London, 1961
Hamperl H: The Myothelia (Myoepithelial Cells). Current Topics Pathol 53: 161–220, 1970
Azzopardi JG: Problems in Breast Pathology, Vol II. WB Saunders, London, 1979
Dairkee SH, Blayney-Moore CM, Smith HS, Hackett AJ: Concurrent expression of basal and luminal epithelial markers in cultures of normal human breast analyzed using monoclonal antibodies. Differentiation 32: 93–100, 1986
Wells S, Hasleton PS: A dilutional immunoperoxidase study of proliferative ductal lesions and carcinomata of the breast. Histopathol 5: 517–526, 1981
Haagensen DE Jr, Mazoujian G, Dilley WG, Pederson CE, Kister SJ, Wells SA Jr: Breast gross cystic disease fluid analysis, I. Isolation and radioimmunoassay for a major component protein. J Natl Cancer Inst 62: 239–247, 1979
Dardick I, Von Nostrand AWP: Myoepithelial cells in salivary gland tumors-revisited. Head and Neck Surg 7: 395–408, 1985
Batsakis JG, Kraemer B, Sciubba J: The pathology of head and neck tumors: the myoepithelial cell and its participation in salivary gland neoplasia, part 17. Head and Neck Surg 5: 222–233, 1983
Dulbecco R, Allen RW, Bologna M, Bowman M: Marker evolution during the development of the rat mammary gland: Stem cells identified by markers and the role of myoepithelial cells. Cancer Res 46: 2449–2456, 1986
Dairkee SH, Mayall BH, Smith HS, Hackett AJ: Monoclonal marker that predicts early recurrence of breast cancer. Lancet i: 54, 1987
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dairkee, S.H., Ljung, B.M., Smith, H. et al. Immunolocalization of a human basal epithelium specific keratin in benign and malignant breast disease. Breast Cancer Res Tr 10, 11–20 (1987). https://doi.org/10.1007/BF01806130
Issue Date:
DOI: https://doi.org/10.1007/BF01806130