Summary
A large proteoglycan with chondroitin sulphate and dermatan sulphate side chains has been isolated and purified from a yolk sac tumour of the left ovary from a 23-year-old female. A monoclonal antibody, designated 2B1, was produced which reacted specifically with the intact molecule of the large proteoglycan and the chondroitinase ABC-treated core molecule. The localization of substances showing cross-reactivity to this antibody was studied in a variety of human tissues by means of indirect immunohistochemistry. The interstitial elements of nearly all tissues of a 5-month-old foetus were intensely reactive with the antibody, but in adult tissues structures that gave positive reactions were limited; only the perivascular and perimuscular fibrous elements were reactive, except for the aorta, which reacted extensively. In contrast, the interstitial elements of the carcinoma tissues tested were intensely reactive. Thus antibody 2B1 can be regarded as a useful tool for studies on the immunohistochemical localization of large proteoglycan in various human tissues.
Similar content being viewed by others
References
BRENNAN, M. J., OLDBERG, A., PIERSCHBACHER, M. D. & RUOSLAHTI, E. (1984) Chondroitin/dermatan sulfate proteoglycan in human fetal membranes. Demonstration of an antigenically similar proteoglycan in fibroblasts.J. Biol. Chem. 259, 13742–50.
CARRINO, D. A. & CAPLAN, A. I. (1982) Isolation and preliminary characterization of proteoglycans synthesized by skeletal muscle.J. Biol. Chem. 257, 14145–54.
CARRINO, D. A. & CAPLAN, A. I. (1984) Isolation and partial characterization of high-buoyant-density proteoglycans synthesizedin ovo by embryonic chick skeletal muscle and heart.J. Biol. Chem. 259, 12419–30.
CÖSTER, L. & FRANSSON, L-Å (1981) Isolation and characterization of dermatan sulphate proteoglycans from bovine sclera.Biochem. J. 193, 143–53.
DAMLE, S. P., COSTER, L. & GREGORY, J. D. (1982) Proteodermatan sulfate isolated from pig skin.J. Biol. Chem. 257, 5523–7.
FISHER, L. W., TERMINE, J. D., DEJTER, S. W., WHITSON, S. W., YANAGISHITA, M., KIMURA, J. H., HASCALL, V. C. KLEINMAN, H. K., HASSELL, J. R. & NILSSON, B. (1983) Proteoglycans of developing bone.J. Biol. Chem. 258, 6588–94.
FUKATSU, T., SOBUE, M., NAGASAKA, T., OHIWA, N., FUKATA, S., NAKASHIMA, N. & TAKEUCHI, J. (1988) Immunohistochemical localization of chondroitin sulphate and dermatan sulphate proteoglycans in tumour tissues.Brit. J. Cancer 57, 74–8.
FUJII, N. & NAGAI, Y. (1981) Isolation and characterization of a proteoglycan sulfate from calf skin.J. Biochem. (Tokyo).90, 1249–59.
HEINEGÅRD, D., BJÖRNE-PERSSON, A., CÖSTER, L., FRANZÉN, A., GARDELL, S., MALMSTRÖM, A., PAULSON, M., SANDFALK, R., & VOGEL, K (1985). The core proteins of large and small interstitial proteoglycans from various connective tissues from distinct subgroups.J. Biochem. 230, 181–94.
HEINEGÅRD, D. & SOMMARIN, Y. (1987) Proteoglycans: an overview.Methods Enzymol. 144, 305–19.
OLDBERG, A., HAYMAN, E. G. & RUOSLAHTI, E. (1981) Isolation of a chondroitin sulfate proteoglycan from a rate yolk sac tumor and immunochemical demonstration of its cell surface localization.J. Biol. Chem. 256, 10847–52.
POOLE, A. R. (1986) Proteoglycans in health and disease: structures and functions.Biochem. J. 236, 1–14.
POOLE, A. R., WEBBER, C., PIDOUX, I., CHOI, H. & ROSENBERG, L. C. (1986) Localization of a dermatan sulfate proteoglycan (DS-PGII) in cartilage and the presence of an immunologically related species in other tissues.J. Histochem. Cytochem. 34, 619–25.
SOBUE, M., FUKATSU, T., NAKASHIMA, N., TAKEUCHI, J., KATOH, T., OGURA, T. & NAKAGAKI, S. (1987a) Immunohistochemical localization of chondroitin sulfate and dermatan sulfate proteoglycan in human connective tissues.Connective Tissue 19, 117–26 (in Japanese).
SOBUE, M., TAKEUCHI, J., YOSHIDA, K., AKAO, S., FUKATSU, T., NAGASAKA, T. & NAKASHIMA, N. (1987b) Isolation and characterization of proteoglycans from human nonepithelial tumors.Cancer Res. 47, 160–8.
SOBUE, M., NAKASHIMA, N., FUKATSU, T., NAGASAKA, T., KATOH, T., OGURA, T. & TAKEUCHI, J. (1988) Production and characterization of monoclonal antibody to dermatan sulfate proteoglycan.J. Histochem. Cytochem. 36, 479–85.
ULDBJERG, N., MALMSTRÖM, A., EKMAN, G., SHEEHAN, J., ULMSTEN, U. & WINGERUP, L. (1983) Isolation and characterization of dermatan sulphate proteoglycan from human uterine cervix.Biochem. J. 209, 497–503.
VOGEL, K. G. & HEINEGÅRD, D. (1985) Characterization of proteoglycans from adult bovine tendon.J. Biol. Chem. 260, 9298–306.
VOSS, B., GLOSSL, J., CULLY, Z. & KRESSE, H. (1986) Immunocytochemical investigation on the distribution of small chondroitin sulfate—dermatan sulfate proteoglycan in the human.J. Histochem. Cytochem. 34, 1013–19.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sobue, M., Nakashima, N., Fukatsu, T. et al. Production and immunohistochemical characterization of a monoclonal antibody raised to proteoglycan purified from a human yolk sac tumour. Histochem J 21, 455–460 (1989). https://doi.org/10.1007/BF01845795
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01845795