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Influence of preoperative core biopsies on uPA/PAI-1 expression in breast cancer tissue

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Abstract

The urokinase-type plasminogen activator (uPA) and the plasminogen activator inhibitor-1 (PAI-1) are involved in tumor invasion and metastasis as well as wound healing and inflammation. We investigated the impact of tissue injury by preoperative sampling on the level of uPA/PAI-1 in paraffin tissue of 55 breast cancer cases by immunohistochemistry. The tumor area surrounding the biopsy channel was compared with distant intact tumor tissue. uPA and PAI-1 were constantly expressed by the tumor cells. Fibroblastic expression was higher in the tumor area surrounding the biopsy channel than in intact tissue with 47 vs 29 positive cases for uPA (p < 0.001) and 35 vs 25 positive cases for PAI-1 (p = 0.055). A decrease in fibroblastic enzyme expression from the biopsy area to the intact tumor tissue was seen in 21 cases for uPA and 16 cases for PAI-1. This difference was most evident in cases with an interval of 6 days and longer between biopsy and surgery. In conclusion, fibroblastic inflammatory reaction around the biopsy channel affects stromal uPA and PAI-1 expression, which possibly leads to falsely increased enzyme levels in ELISA. Tissue specimen for ELISA analysis should not be taken from the tumor area around the biopsy channel in the resection specimen.

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References

  1. Andreasen PA, Egelund R, Petersen HH (2000) The plasminogen activation system in tumor growth, invasion, and metastasis. Cell Mol Life Sci 57(1):25–40

    Article  PubMed  CAS  Google Scholar 

  2. Andreasen PA, Kjoller L, Christensen L, Duffy MJ (1997) The urokinase-type plasminogen activator in cancer metastasis: a review. Int J Cancer 72:1–22

    Article  PubMed  CAS  Google Scholar 

  3. Camps JL, Chang SM, Hsu TC, Freeman MR, Hong SJ, Zhau HE, von Eschenbach AC, Chung LW (1990) Fibroblast mediated acceleration of human epithelial tumor growth in vivo. Proc Natl Acad Sci USA 87:75–79

    Article  PubMed  CAS  Google Scholar 

  4. Dublin E, Hanby A, Patel NK, Liebman R, Barnes D (2000) Immunohistochemical expression of uPA, uPAR, and PAI-1 in breast carcinoma. Am J Pathol 157:1219–1227

    PubMed  CAS  Google Scholar 

  5. Duffy MJ, Reilly D, O’Sullivan C, O’Higgins N, Fennelly JJ, Andreasen P (1990) Urokinase-plasminogen activator, a new and independent prognostic marker in breast cancer. Cancer Res 50:6827–6829

    PubMed  CAS  Google Scholar 

  6. Ferrier CM, de Witte HH, Straatman H, van Tienoven DH, van Geloof WL, Rietveld FJR, Sweep CGJ, Ruiteer DJ, van Muijen GNP (1999) Comparison of immunohistochemistry with immunoassay (ELISA) for the detection of components of the plasminogen activation system in human tumour tissue. Br J Cancer 79:1534–1541

    Article  PubMed  CAS  Google Scholar 

  7. Ganesh S, Sier CF, Griffioen G, Vloedgraven HJ, de Boer A, Welvaart K, de Velde CJ, van Krieken JH, Verheijen JH, Lamers CB (1994) Prognostic relevance of plasminogen activators and their inhibitors in colorectal cancer. Cancer Res 54:4065–4071

    PubMed  CAS  Google Scholar 

  8. Harbeck N, Kates RE, Look MP, Meijer-van Gelder ME, Klijn JGM, Jänicke F, Krüger A, Kiechle M, Schmitt M, Foekens JA (2002) Enhanced benefit from adjuvant systemic chemotherapy in breast cancer patients classified high risk according to uPA and PAI-1 (n = 3424). Cancer Res 62:4617–4622

    PubMed  CAS  Google Scholar 

  9. Jänicke F, Prechtl A, Thomssen C, Harbeck N, Meisner C, Untch M, Sweep CG, Selbmann HK, Graeff H, Schmitt M, for the German Chemo N0 Study group (2001) Randomized adjuvant chemotherapy trial in high-risk lymph node-negative breast cancer patients identified by urokinase-type plasminogen activator and plasminogen activator inhibitor type I. J Natl Cancer Inst 93:913–920

    Article  PubMed  Google Scholar 

  10. Lee AHS, Gillett CE, Ryder K, Fentiman IS, Miles DW, Millis RR (2006) Different patterns of inflammation and prognosis in invasive carcinoma of the breast. Histopathology 48:692–701

    Article  PubMed  CAS  Google Scholar 

  11. Liotta LA, Kohn EC (2001) The microenvironment of the tumour–host interface. Nature 411:375–379

    Article  PubMed  CAS  Google Scholar 

  12. Look MP, van Putten WLJ, Duffy MJ, Harbeck N, Christensen IJ, Thomssen C, Kates R, Spyratos F, Fernö M, Eppenberger-Castori S, Sweep CGJ, Ulm K, Peyrat JP, Martin PM, Magdelenat H, Brünner N, Duggan C, Lisboa BW, Bendahl PO, Quillien V, Daver A, Ricolleau G, Meijer-van Gelder ME, Mander P, fiets E, Blankenstein MA, Broet P, Romain S, Daxenbichler G, Windbichler G, Cufer T, Borstnar S, Kueng W, Beex LVAM, Klijn JGM, O’Higgins NO, Eppenberger U, Jänicke F, Schmitt M, Foekens JA (2002) Pooled analysis of prognostic impact of urokinase-type plasminogen activator and its inhibitor PAI-1 in 8377 breast cancer patients. J Natl Cancer Institute 94:116–128

    CAS  Google Scholar 

  13. Nielsen BS, Sehested M, Suun S, Rank F, Timshel S, Rygaard J, Johnsen M, Dano K (2001) Urokinase plasminogen activator is localized in stromal cells in ductal breast cancer. Lab Invest 81:1485–1501

    PubMed  CAS  Google Scholar 

  14. Olumi AF, Dazin P, Tlsty TD (1998) A novel coculture technique demonstrates that normal human prostatic fibroblasts contribute to tumor formation of LNCaP cells by retarding cell death. Cancer Res 58:4525–4530

    PubMed  CAS  Google Scholar 

  15. Pappot H, Gardsvoll H, Romer J, Pedersen AN, Grondahl-Hansen J, Pyke C, Brunner N (1995) Plasminogen activator inhibitor type 1 in cancer: therapeutic and prognostic implications. Biol Chem Hoppe Seyler 376(5):259–267

    PubMed  CAS  Google Scholar 

  16. Pyke C, Ralfkiaer E, Tryggvason K, Dano K (1993) Messenger RNA for two type IV collagenases is located in stromal cells in human colon cancer. Am J Pathol 142:359–365

    PubMed  CAS  Google Scholar 

  17. Schäfer BM, Maier K, Eickhoff U, Todd RF, Kramer MD (1994) Plasminogen activation in healing human wounds. Am J Pathology 144:1269–1280

    Google Scholar 

  18. Schmitt M, Janicke F, Moniwa N, Chucholowski N, Pache L, Graeff H (1992) Tumor-associated urokinase-type plasminogen activator: biological and clinical significance. Biol Chem Hoppe Seyler 373(7):611–22

    PubMed  CAS  Google Scholar 

  19. Sweep CGJ, Geurts-Moespot J, Grebenschikov N, de Witte JH, Heuvel JJTM, Schmitt M, Duffy MJ, Jänicke F, Kramer MD, Foekens JA, Brünner N, Brugal G, Pedersen AN, Benraad TJ (1998) External quality assessment of trans-European multicentre antigen determinations (ELISA) of urokinase-type plasminogen activator (uPA) and its type-1 inhibitor (PI-1) in human breast cancer tissue extracts. Br J Cancer 78:1434–1441

    PubMed  CAS  Google Scholar 

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We declare that we have no conflict of interest.

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Authors and Affiliations

  1. Institute of Pathology, Sigmund Freud Str. 25, D-53127,, Bonn, Germany

    Susanne Haas, Jens Claus Hahne & Hans-Peter Fischer

  2. Department of Obstetrics and Gynecology, Medical Faculty of the University of Bonn, Bonn, Germany

    Tjoung-Won Park

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  1. Susanne Haas
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  2. Tjoung-Won Park
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  3. Jens Claus Hahne
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  4. Hans-Peter Fischer
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Correspondence to Susanne Haas.

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Haas, S., Park, TW., Hahne, J.C. et al. Influence of preoperative core biopsies on uPA/PAI-1 expression in breast cancer tissue. Virchows Arch 452, 277–283 (2008). https://doi.org/10.1007/s00428-007-0563-8

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  • DOI: https://doi.org/10.1007/s00428-007-0563-8

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