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Isolation and characterization of new microsatellites at the nm23-H1 and nm23-H2 gene loci and application for loss of heterozygosity (LOH) analysis

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Abstract

 The nm23-H1 gene has been suggested to be a metastasis suppressor gene. Studies about the events of loss of heterozygosity (LOH) at the nm23 locus and its correlation to metastasis are controversially discussed. To optimize detection of LOH at the nm23 locus, we screened two P1 clones for additional microsatellites. Tumor and normal DNA from 37 colorectal, 16 gastric, and 8 germ cancer patients were examined for LOH. We found two new CA repeats, one 5′ to nm23-H1 and another 3′ to nm23-H2. Using these nm23 locus-specific CA repeats and five other chromosome 17 loci (D17S1522, D17S1566, D17S855, D17S515, and TP53), allele loss was observed in 4/32 (12.5%) patients with colon cancer, 2/14 (14.3%) with gastric cancer, and 1/7 (14%) with germ cancer. No isolated LOH of the nm23 region was observed.

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Authors and Affiliations

  1. Institut für Humangenetik, Universität des Saarlandes, Gebäude 68, D-66421 Homburg/Saar Germany, Tel.: +49 (0)6841166601, Fax: +49 (0)6841166600, e-mail: hgsdoo@med-rz.uni-sb.de, , , , , , DE

    Markus Seifert, Birgit Theisinger, Matthias Engel, Thomas Seib, Karin Hilgert & C. Welter

  2. Institut für Pathologie, Klinikum Bamberg, D-96049 Bamberg, Germany, , , , , , DE

    Gerhardt Seitz

  3. Institut für Pathologie, Klinikum Bayreuth, D-95445 Bayreuth, Germany, , , , , , DE

    Manfred Stolte

Authors
  1. Markus Seifert
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  2. Birgit Theisinger
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  3. Matthias Engel
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  4. Thomas Seib
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  5. Gerhardt Seitz
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  6. Manfred Stolte
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  7. Karin Hilgert
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  8. C. Welter
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Received: 5 May 1997 / Accepted: 2 June 1997

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Seifert, M., Theisinger, B., Engel, M. et al. Isolation and characterization of new microsatellites at the nm23-H1 and nm23-H2 gene loci and application for loss of heterozygosity (LOH) analysis. Hum Genet 100, 515–519 (1997). https://doi.org/10.1007/s004390050545

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  • DOI: https://doi.org/10.1007/s004390050545

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