Influence of collection and separation of blood samples on plasma IL-1, IL-6 and TNF-α concentrations
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2021, The Lancet Respiratory MedicineCitation Excerpt :Factors relating to the processing and handling of samples also stand to influence assay measurements.61–65 Delays in processing are particularly relevant, since IL-6 and other cytokines are released spontaneously from blood cells over time or after the priming of these cells in samples that are agitated or shaken when being transported to the laboratory.61,62 This makes the practice of processing blood samples in batches after long periods of time in storage at room temperature unsuitable for cytokine studies.64
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