Integration of human papillomavirus type 16 into cellular DNA of cervical carcinoma: Preferential deletion of the E2 gene and invariable retention of the long control region and the E6/E7 open reading frames

doi:10.1016/0042-6822(87)90195-4

Virology

Volume 161, Issue 1, November 1987, Pages 259-261

https://doi.org/10.1016/0042-6822(87)90195-4 Get rights and content

Abstract

The integration patterns of human papillomavirus (HPV) type 16 in the cellular DNA of six cervical carcinoma samples were analyzed by the Southern blot procedure. None of the HPV integrants retained the entire viral genome. Double HPV integration was found in one case while all other cases were single integrants. In some samples, internal deletion and selective amplification of the viral sequences were observed. On integration, the E2 open reading frame (ORF) was invariably lost but the E6/E7 ORFs and the long control region of the HPV-16 genome were retained in all seven integrations analyzed and may play a role in cellular transformation and/or maintainence of the transformed phenotype.

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Citation Excerpt :
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Short communicationIntegration of human papillomavirus type 16 into cellular DNA of cervical carcinoma: Preferential deletion of the E2 gene and invariable retention of the long control region and the E6/E7 open reading frames

Abstract

Virology

Virology

Cell

Rev. Physiol. Biochem. Pharmacol.

Nature (London)

J. Virol.

Nature (London)

EMBO J.

J. Med. Virol.

N. Engl. J. Med.

J. Virol.

Short communication
Integration of human papillomavirus type 16 into cellular DNA of cervical carcinoma: Preferential deletion of the E2 gene and invariable retention of the long control region and the E6/E7 open reading frames