[36] Phagocytic leukocyte oxygenation activities and chemiluminescence: A kinetic approach to analysis

doi:10.1016/0076-6879(86)33085-4

Methods in Enzymology

Volume 133, 1986, Pages 449-493

https://doi.org/10.1016/0076-6879(86)33085-4 Get rights and content

Publisher Summary

This chapter discusses a kinetic approach to analysis of phagocytic leukocyte oxygenation activities and chemiluminescence. Chemiluminescence (CL) is an energy product of phagocyte oxygenation activity. Measurement of this luminescence is nondestructive and allows continuous monitoring of phagocyte oxygenation activity. As an end product of the microbe-humoral-phagocyte interaction, phagocyte luminescence may also be used for assessing the functional interrelationships of the various reacting components. For example, when stimulus is not limiting, CL is proportional to the functional capacity of the phagocytes present. Likewise, if the number of phagocytes is not limiting, microbe opsonification kinetics can be studied as the rate of stimulated phagocyte oxygenation activity yielding CL. Thus, the CL approach can provide an in vitro model system for analysis of the humoral immune mechanisms. Both native CL and chemiluminigenic probe-dependent CL techniques are successfully employed for assessment of PMNL and monocyte-macrophage oxygenation activities in a number of laboratories.

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