Original article
Mucosal antibody response to parenteral vaccination with Haemophilus influenzae type b capsule

https://doi.org/10.1016/0091-6749(83)90585-7Get rights and content

Abstract

The simultaneous serum and mucosal antibody response to parenteral vaccination with the Haemophilus influenzae type b (Hib) polysaccharide capsule (PRP) was evaluated in a group of 10 children and nine adults. All subjects responded to parenteral vaccination with an increase in serum anticapsular antibody. The children's preimmunization anti-PRP antibody level (x= 0.04 μg/ml) and 3 wk postimmunization level (x = 19.3 μg/ml) were lower than the adults' (preimmunization x = 1.5 μg/ml; postimmunization x = 81.2 μg/ml). Eight of 10 children and seven of nine adults also developed a rise in antibody in nasal secretions. The children's mean nasal preimmunization level was 0.74 μg/mg IgA and mean postimmunization level was 5.0 μg/mg IgA. The adults' mean nasal preimmunization level was 0.98 μg/mg IgA and mean postimmunization level was 3.0 μg/mg IgA. Salivary antibody responses generally followed the pattern of nasal antibody responses. These data suggest that parenteral administration with the Hib capsular polysaccharide can produce a mucosal antibody response. Furthermore, although serum antibody responses to PRP vaccination are greater in adults than in children, mucosal antibody responses are comparable.

References (33)

  • TB Tomasi et al.

    Mucosal immunity: the origin and migration patterns of cells in the secretory system

    J Allergy Clin Immunol

    (1980)
  • DC Turk et al.

    Haemophilus influenzae: its clinical importance

    (1967)
  • HE Alexander et al.

    The protective or curative element in type b H. influenzae rabbit serum

    Yale J Biol Med

    (1944)
  • R Schneerson et al.

    Immunity to disease caused by Haemophilus influenzae type b. II. Specificity and some biologic characteristics of “natural” infection acquired and immunization induced antibodies to the capsular polysaccharide of Haemophilus influenzae type b

    J Immunol

    (1971)
  • P Anderson et al.

    Immunization of humans with polyribophosphate, the capsular antigen of Haemophilus influenzae type b

    J Clin Invest

    (1972)
  • H Peltola et al.

    Haemophilus influenzae type b capsular polysaccharide vaccine in children: a double-blind field study of 100,000 vaccinees 3 months to 5 years of age in Finland

    Pediatrics

    (1977)
  • ME Pichichero et al.

    A mucosal antibody response following systemic Haemophilus influenzae type b infection in children

    J Clin Invest

    (1981)
  • ME Pichichero et al.

    Relationship between naturally occurring human mucosal and serum antibody to the capsular polysaccharide of Haemophilus influenzae type b

    J Infect Dis

    (1982)
  • P Anderson et al.

    Isolation of the capsular polysaccharide from culture supernatant of Haemophilus influenzae type b

    Infect Immun

    (1976)
  • KR Powell et al.

    Improved method of collection of nasal mucus

    J Infect Dis

    (1977)
  • ME Schaefer et al.

    A plastic intraoral device for the collection of human parotid saliva

    J Dent Res

    (1977)
  • RB Newman et al.

    Latex agglutination test for the diagnosis of Haemophilus influenzae meningitis

    J Lab Clin Med

    (1970)
  • RH Michaels et al.

    Use of antiserum agar for detection of Haemophilus influenzae type b in the pharynx

    Pediatr Res

    (1975)
  • P Anderson

    Intrinsic tritium labelling of the capsular polysaccharide antigen of Haemophilus influenzae type b

    J Immunol

    (1978)
  • JB Robbins et al.

    Quantitative measurement of “natural” and immunization induced Haemophilus influenzae type b capsular polysaccharide antibodies

    Pediatrics

    (1973)
  • ME Pichichero et al.

    Breast milk antibody to the capsular polysaccharide of Haemophilus influenzae type b

    J Infect Dis

    (1979)
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    This work was supported by U.S.P.H.S. grant AI 17217 and by contract AI 72523 from the National Institute of Allergy and Infectious Diseases.

    1

    Dr. Pichichero was a fellow under National Institutes of Health training grant AI 07169.

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