A family of serine esterases in lytic granules of cytolytic T lymphocytes

doi:10.1016/0092-8674(87)90544-7

Cell

Volume 49, Issue 5, 5 June 1987, Pages 679-685

https://doi.org/10.1016/0092-8674(87)90544-7 Get rights and content

Abstract

Cytoplasmic granules of cytolytic T lymphocytes (CTLs) contain, in addition to the pore-forming protein perforin, a family of highly homologous serine esterases, granzymes A-H. The serine esterase affinity label diisopropyl fluorophosphate reacts strongly with granzymes A and D, to a lesser extent with B, E, F, G, and H, and not at all with C and F. For granzymes A and D, synthetic substrates have been found. Antibodies raised against granzyme B strongly cross-react with A, G, and H, and antibodies to granzyme D recognize C, E, and F. These antigenic relationships correlate with similarities in the N-terminal amino acid sequences. At least 60% homology is observed between the eight proteins, and all are similar to rat mast cell protease 2. Sequence analysis suggests the identity of granzyme A with a protease predicted from a CTL-specific cDNA clone (H factor) and of granzyme B, G, or H with a protein encoded by the CTL-specific cDNA clone CTLA 1/CCP 1.

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Citation Excerpt :
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Cell

ArticleA family of serine esterases in lytic granules of cytolytic T lymphocytes

Abstract

J. Biol. Chem.

Anal. Biochem.

Anal. Biochem.

J. Biol. Chem.

FEBS Lett.

Immunol. Today

Immunol. Today

Cell

The inducible cytotoxic T-lymphocyte-associated gene transcript CTLA-1 sequence and gene localization to mouse chromosome 14

Nature

Effects of Nα-tosyl-l-lysylchloromethyl-ketone on the activity of cytotoxic T lymphocytes

J. Immunol.

Cloning of a cDNA for a T cell-specific serine protease from a cytotoxic T lymphocyte

Science

Mechanism of lymphocyte-mediated cytotoxicity

Ann. Rev. Immunol.

Cleavage of structural proteins during the assembly of the head of bacteriophage T4

Nature

The mechanism of human NK cell-mediated cytotoxicity

Mode of action of surface-associated proteases in the earlier stages of the lytic reaction

J. Immunol.

Cloning of two genes that are specifically expressed in activated cytotoxic T lymphocytes

Article
A family of serine esterases in lytic granules of cytolytic T lymphocytes