Detection by PCR of HHV-6 and EBV DNA in blood and oropharynx of healthy adults and HIV-seropositives
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Cited by (167)
Epstein-Barr virus infection in gliomas
2019, Current Research in Translational MedicineCitation Excerpt :The presence of amplifiable DNA was verified through amplification of a 268-bp sequence of the human β-globin gene using a set of primers as described [32]. PCR was carried out using a primer set, allowing the amplification of a 175-bp fragment of the EBV BamM region (sense: 5′AACATGCTGTATGCCTCGCAGCG-3′; antisense: 5′AATTACTGGCGTGAATTGTGCCCA-3) [33]. The PCR reactions were performed using 200 ng of DNA template in a total volume of 25 μl as described previously [32].
Fulminant hepatic failure attributed to infection with human herpesvirus 6 (HHV-6) in an immunocompetent woman: A case report and review of the literature
2016, Journal of Clinical VirologyCitation Excerpt :HHV-6 has rarely been implicated as a cause of non-A, non-B, non-C (NABC) hepatitis in immunocompetent adults [13,19,24]. HHV-6 has been detected in saliva and PBMC of healthy individuals, with prevalences of 8–95% [11,16,23]. However, higher levels of HHV-6 DNA are detected in symptomatic patients [3,15,26].
Pretransplantation Evaluation: Infectious Disease
2015, Transplantation of the Liver: Third EditionEvaluation of multiplex polymerase chain reaction and microarray-based assay for rapid herpesvirus diagnostics
2012, Diagnostic Microbiology and Infectious DiseaseInvestigation of Epstein-Barr virus in breast carcinomas in Tunisia
2011, Pathology Research and PracticeCitation Excerpt :Extracted DNAs were assessed for their suitability for PCR analysis by a control reaction designed to amplify a fragment of the 407-base paired (bp), of the β-globin gene as described previously [33]. The presence of EBV was investigated by a PCR protocol using a specific set of primers EBV-S (3′-AACATGCTGTATGCCTCGCAGCG-5′) and EBV-R (3′-AATTACTGGCGTGAATTGTGCCCA-5′) amplifying a 176-bp sequence in the BamHI G region of the EBV genome [15]. The PCR reactions were performed using 200 ng of DNA template in a total volume of 25 μl, containing 0.2 μM of each sense and antisense primers, 10 mM Tris–HCl (pH 8.4), 50 mM KCl, 1.5 mM MgCl2, 200 mM of each dNTP, and 1 U of Taq DNA polymerase (Promega, Madison, USA) in a PTC 200™ DNA engine thermal cycler (MJ Research, Watertown, USA).
Primary Human Herpesvirus-6 Infection in the Central Nervous System Can Cause Severe Disease
2007, Pediatric NeurologyCitation Excerpt :After discharge, they were followed by a child neurologist for 4 to 7 years. Total DNA was extracted by phenol-chloroform from 100 μL of cerebrospinal fluid and amplified in a 100-μL reaction mixture containing 50 mM KCl, 10 mM Tris-HCl (pH 8.3), 1.5 mM MgCl, 0.01% (w/v) gelatin, 200 μM each of the four deoxyribonucleoside triphosphates, 2.5 units of AmpliTaq Gold polymerase (Applied Biosystems, Foster City, CA), and 50 pmol of both primers [21], chosen from the U67 gene (5′-AAG CTT GCA CAA TGC CAA AAA ACA G-3′ and 5′-biotin-CTC GAG TAT GCC GAG ACC CCT AAT C-3′). The target sequence was amplified with the following thermal profile: step 1, 95°C for 10 minutes; step 2, 95°C for 30 seconds, 51°C for 30 seconds, and 72°C for 1 minute, for 40 cycles; and step 3, 72°C for 10 minutes.