Vimentin and keratin intermediate filaments expression by K562 leukemic cell line

doi:10.1016/0145-2126(86)90102-5

Leukemia Research

Volume 10, Issue 1, 1986, Pages 29-33

https://doi.org/10.1016/0145-2126(86)90102-5 Get rights and content

Abstract

The expression of intermediate filaments (IMF) by K562 leukemic cell line has been studied by the use of polyclonal and monoclonal antibodies. These cells exhibited an extremely rich network of IMF reacting with both anti-vimentin and anti-keratin antibodies.

Although these results need to be confirmed by different techniques, such as immunoblotting, they suggest the possibility that leukemic cell lines can express different types of IMF similarly to other cultured cells.

References (10)

E. Linder et al.
Autoantibody to intermediate filaments in infectious mononucleosis
Clin. Immun. Immunopath.
(1979)
R.M. Pruss et al.
All classes of intermediate filaments share a common antigenic determinant defined by a monoclonal antibody
Cell
(1981)
K. Dellagi et al.
Alteration of vimentin intermediate filaments expression during differentiation of HL60 and U937 human leukemic cell lines
Leukemia Res.
(1984)
B.B. Lozzio et al.
Human chronic myelogenous leukemia cell line with positive Philadelphia chromosome
Blood
(1975)
D. Zauli et al.
Detection of anti-cytoskeleton antibodies using vinblastinetreated mononuclear cells
J. Immun. Methods
(1985)

There are more references available in the full text version of this article.

Cited by (11)

Vimentin is a component of a complex that binds to the 5′-UTR of human heme-regulated eIF2α kinase mRNA and regulates its translation
2013, FEBS Letters
Citation Excerpt :
The ∼100 kDa band, which was also seen in the UV crosslinking experiments could not be identified with confidence, while the 55 kDa protein band was identified as human vimentin. Vimentin is a type III intermediate filament (IF) protein and is expressed in K562 cells [14]. We envisaged that it may be the 55 kDa protein, as observed earlier, in UV-crosslinking studies (Fig. 4C).
The human heme-regulated eIF2α kinase, also called the human heme-regulated inhibitor (hHRI) is significantly up-regulated particularly at the level of translation during stress. In this report we show that during lead-stress, the regulation of hHRI mRNA translation is mediated through its 5′-untranslated region (UTR) that interacts with specific trans-acting factors. Further, vimentin has been identified as one of the trans-acting factors that contribute to this regulation.
Selection and characterization of an erythroeosinophilic subclone (LAMA-87) and an eosinophilic subclone (LAMA-88) from the multipotential cell line LAMA-84
1994, Leukemia Research
The human leukemic cell line LAMA-84 was established and characterized as an erythromegakaryocytic cell line. In the present study we show that these cells can differentiate in estrone-treated athymic mice and give rise to an erythroeosinophilic cell line (LAMA-87). This new cell line expressed glycophorin A, α β and γ globin chain mRNA but also eosinophilic peroxidase. Hemin slightly increased the total hemoglobin production of the cells and phorbol diester (TPA), dimethyl sulfoxide (DMSO) and sodium butyrate (SB) increased the expression of megakaryocytic markers (gpllb/IIIa complex). When inoculated into non-treated athymic mice, LAMA-87 cells can differentiate to give rise to eosinomonocytic cells (LAMA-88). This new cell line expresses eosinophilic peroxidase, Luxol fast blue stain and synthesizes lysozyme. Depending on the inducer used, LAMA-88 can differentiate along a monocytic lineage (TPA, DMSO, SB and vitamin D3).
These three LAMA cell lines should be useful in further studies of the molecular regulation of the pluripotent cell commitment and may provide a model for the understanding of human hematopoiesis.
Cytokeratins and cytokeratin filaments in subpopulations of cultured human and rodent cells of nonepithelial origin: modes and patterns of formation
1989, Differentiation
Using immunofluorescence microscopy, we observed that in several established cell culture lines derived from different nonepithelial tissues and species, cells spontaneously emerge, usually at low frequencies, which contain cytoplasmic structures decorated by antibodies specific for cytokeratins 8 and 18. This phenomenon was further examined at both the protein (gel electrophoreses of cytoskeletal proteins, followed by immunoblotting) and the RNA (Northern blots, “nuclear run-on” analysis, in situ hybridization) level. Positive cell lines included simian virus (SV40)-transformed human fibroblasts (HF-SV80, WI-38 VA13), human astrocytic glioma cells (U333 CG/343MG), rat (RVF-SMC) and hamster (BHK-21/13) cells derived from vascular smooth muscle and murine sarcoma MS-180 cells. In two cell lines (HF-SV80 and BHK-21/13), the frequency of the cytokeratin-containing cells and of the cytokeratin fibril arrays per cell was drastically increased upon treatment with 5-azacytidine. The structural appearance of the cytokeratins was variable in the different cell lines but could also differ among cells of the same culture: While small granular or comma-shaped structures or bizarrely shaped filament arrays prevailed in WI-38, RVF and normally grown BHK-21 cells, most of the other lines revealed extended normal-looking, fibrillar arrays. In one line (MS-180), the appearance of cytokeratins was associated with a morphological change, as it was only found in a subpopulation of cells that had lost their typical elongated and spindle-shaped phenotype and assumed a rounded (“coccoid”) shape. Our results show that the expression of the genes encoding cytokeratins 8 and 18 is not necessarily restricted to programs of epithelial differentiation and that factors stochastically effective appear in cultured cell lines that allow the synthesis of these cytoskeletal components. Mechanisms possibly involved in this spontaneous and selective advent of cytokeratins 8 and 18 and implications for tumor diagnosis are discussed.
Cytokeratin analysis of breast and leukemia tumor cell lines by flow cytometry
1989, Biology of the Cell
Using four human tumor cell lines, MCF-7 and T47-D from breast tumors, MOLT-4 and K-562 from leukemia, flow cytometric DNA analysis of pure and mixed cell population was performed using monoclonal antibodies to cytokeratin to distinguish cytokeratin-containing carcinoma cells from leukemia cells which do not contain cytokeratins. Surprisingly, on pure or mixed K-562 cells, we found positive labeling with KL1, CK8, and CK18 antibodies (results confirmed by immunocytology). This preliminary study has allowed a DNA analysis on epithelial cells of human breast tumors.
Transient coexpression of desmin and cytokeratins 8 and 18 in developing myocardial cells of some vertebrate species
1988, Differentiation
During myogenesis the intermediate-sized filament (IF) cytoskeleton is characterized by increasing proportions of desmin. While skeletal and smooth muscle formation occurs in free mesenchymal cells containing vimentin-type IFs, myocardial development starts from a polar epithelium containing cytokeratin IFs and desmosomes. Therefore, we have studied the formation of the epicardium and the myocardium in different vertebrate species, combining light and electron microscopic immunolocalization techniques with gel-electrophoretic analyses of cytoskeletal proteins of microdissected myocardial tissue at differing developmental stages. In this report, we describe results obtained from advanced stages of myocardial differentiation. In all species studied the myocardial cell possess IFs abundant in desmin, often together with smaller amounts of vimentin, and the mesothelial layer of the epicardium contains cytokeratin IFs. However, we have observed remarkable interspecies differences with respect to the occurrence of cytokeratins in embryonic myocardial cells. In fetal human myocardium, from week 10 of pregnancy on, but not in juvenile and adult myocardium, and in chicken myocardium of all stages examined (until several days after hatching) specific immunostaining was seen with certain broad-range cytokeratin antibodies as well as with antibodies specific for cytokeratins 18 (in both species) and 8 (showing significant reaction only in human). This cytokeratin immunoreaction, however, did not appear in IFs extending throughout the cytoplasm or at Z-lines, but was localized in punctate arrays representing aggregates of dense material. The aggregates were often enriched at, but not restricted to, the desmosomal plaques of the intercalated discs. These observations were supported by gel-electrophoretic demonstration of small but significant amounts of cytokeratins 18 (in both species) and 8 (detected only in human) in microdissected myocardial tissue. We also observed cytokeratins in smooth muscle cells of some cardiac blood vessels. In contrast, bovine myocardium of advanced fetal age as well as rat and mouse myocardium (from fetal day 12 on) were negative for cytokeratins with all methods, although epicardial cytokeratin IFs were demonstrable. These observations are discussed in relation to myocardial histogenesis and to general problems of cytokeratin gene expression control in epithelial and nonepithelial cells.
Mesenchymal-epithelial transitions: Spontaneous and cumulative syntheses of epithelial marker molecules and their assemblies to novel cell junctions connecting human hematopoietic tumor cells to carcinomatoid tissue structures
2011, International Journal of Cancer

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Vimentin and keratin intermediate filaments expression by K562 leukemic cell line

Abstract

Clin. Immun. Immunopath.

Cell

Leukemia Res.

Human chronic myelogenous leukemia cell line with positive Philadelphia chromosome

Blood

Detection of anti-cytoskeleton antibodies using vinblastinetreated mononuclear cells

J. Immun. Methods