Original contributionInterlaboratory reproducibility of semiautomated cell cycle analysis of flow cytometric DNA-histograms obtained from fresh material of 1,295 breast cancer cases☆
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Aneuploidy identifies subsets of patients with poor clinical outcome in grade 1 and grade 2 breast cancer
2015, BreastCitation Excerpt :Owing to its consistent prognostic information, related to tumour intrinsic biological aggressiveness, the histological grading has been incorporated on the primary treatment of early breast cancer [2], being an additional parameter for deciding the adjuvant therapy to apply in the individual patient. Although still controversial and not routinely used [3,4], due mainly to differences in studies' design, type of material and lack of standardisation [5,6], flow cytometric DNA ploidy has been mostly considered a valuable prognostic factor in breast cancer [7,8]. Our group, studying two large series of patients either with short-term [9] or long-term follow-up [10], showed that aneuploidy is independently associated with unfavourable outcome.
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2003, Gastrointestinal Endoscopy Clinics of North AmericaPredictors of progression to cancer in Barrett's esophagus: Baseline histology and flow cytometry identify low- and high-risk patient subsets
2000, American Journal of GastroenterologyCitation Excerpt :There is an extensive body of literature on reducing sources of interlaboratory variation in flow cytometry, including sample handling, preparation, instrumentation, computer analysis, and histogram interpretation (50–52). Recent studies have shown that flow cytometry can be reproducible; for example, one large study found 94% interlaboratory agreement in a five-tiered classification system of ploidy (53). We are optimistic that interlaboratory differences in instrumentation will not be insurmountable, as we have found excellent agreement between ICP-22 (Partec GmbH, Münster, Germany) and Coulter Elite instruments (Beckman Coulter, Fullerton, CA) (data not shown).
Antitumor effect of GnRH agonist in epithelial ovarian cancer
1999, Gynecologic Oncology
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Supported in part by grant nos. 28-1814 and 28-1398 of the Praeventiefonds.