Short reportsPhage libraries for generation of clinically useful antibodies
References (7)
- et al.
Selection of phage antibodies by binding affinity: mimicking affinity maturation
J Mol Biol
(1992) Targeted therapies: cell surface targets
- et al.
Phage antibodies: filamentous phage displaying antibody variable domains
Nature
(1990)
Cited by (166)
Nanoparticles and trained immunity: Glimpse into the future
2021, Advanced Drug Delivery ReviewsRapid de novo generation of antigen specific human B cells with expression of Blimp‐1 and AID by in vitro immunization
2017, Experimental Cell ResearchCitation Excerpt :It was shown that repeated injections of non-human antibodies led to a strong immune response that significantly limited or undermined their therapeutic efficacy [2,4,6]. Hence, full human mAbs have since emerged as a result of several approaches, including the phage display method and transgenic mouse system with an entire human antibody repertoire [7–12]. However, transgenic mouse system is lengthy and costly.
Plasma half-life extension of small recombinant antibodies by fusion to immunoglobulin-binding domains
2012, Journal of Biological ChemistryCitation Excerpt :Results are shown as mean value ± S.D. ScDb-IgBD and scFv-IgBD fusion proteins were generated by fusing IgBDs from SpA, SpG, or PpL to the C terminus of a bispecific single-chain diabody directed against CEA and CD3 (scDb CEACD3) or a single-chain Fv directed against CEA (scFv CEA) (17) (Fig. 2a). From SpA we used domain B (18), domain D (9), and a modified version of domain E, which was described to exhibit increased thermal stability (19).
Establishing a knowledge trail from molecular experiments to clinical trials
2011, New BiotechnologyCitation Excerpt :Data from experiments to characterize MFE-CP in terms of yield, catalytic activity, integrity and identity are outside GIATE domain. Information on cloning, expression and purification of the murine MFE-23 [38] and humanized MFE-23 crystal structure [35] are not addressed in GIATE. Similarly, properties of experiments with gene fusion to create the protein [39] and subsequent efforts to introduce post-translational modifications by expression in Pichia Pastoris [34] are not addressed.
Convergent potency of internalized gelonin immunotoxins across varied cell lines, antigens, and targeting moieties
2011, Journal of Biological Chemistry