Characterization of a tumor-associated gene, a member of a novel family of genes encoding membrane glycoproteins

doi:10.1016/S0378-1119(96)00475-1

Gene

Volume 183, Issues 1–2, 1996, Pages 69-75

https://doi.org/10.1016/S0378-1119(96)00475-1 Get rights and content

Abstract

To isolate genes involved in tumor formation and in embryogenesis, a subtracted cDNA library was constructed from a c-myc-induced mouse brain tumor. A gene isolated in this screen, named TMP (tumor-associated membrane protein), codes for a putative glycoprotein with four transmembrane domains. The TMP gene was found to be highly expressed in brain tumor cells but not in normal brain. It is also expressed at high levels in undifferentiated embryonic stem cells, but markedly down-regulated in these cells after their differentiation into embryoid bodies. The TMP amino acid sequence bears high homology to the growth arrest specific protein PMP22/GAS-3, which is involved in several human peripheral neuropathies. The expression patterns of the TMP and PMP22 genes in NIH-3T3 fibroblasts were compared at different proliferation states. The results suggest an inverse pattern of expression for the two homologs, TMP expression being high during cell proliferation and PMP22 expression being high during growth arrest. To further characterize the TMP gene we have isolated its human homolog and examined its expression in embryonic and adult tissues. In our search for human sequences homologous to TMP and PMP22, we identified two new genes which we have named XMP and YMP. Thus, we present a novel family of membrane glycoproteins, one member of which is closely associated with proliferation and another with growth arrest.

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Characterization of a tumor-associated gene, a member of a novel family of genes encoding membrane glycoproteins

Abstract

J. Mol. Biol.

Cell

Cell

Anal. Biochem.

Cell

Exp. Eye Res.

Cell

J. Biol. Chem.

Trends Genet.

Trends Neurosci.

Cell

J. Mol. Biol.

J. Biol. Chem.

Transgenic models of tumor development

Science

Hypermyelination and demyelinating peripheral neuropathy in Pmp22-deficient mice

Nature Genet.

Enzymatic amplification of RNA by PCR

Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose

PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates

BK1: an FGF-responsive central nervous system-derived cell line

Growth Factors