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Telomerase activation and incidence of HPV in human gastrointestinal tumors in North Indian population

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Abstract

The present study was initiated with the objective of finding out the role of possible factors in the etiology of gastrointestinal tract cancers. HPV-DNA was detected in 62.5% (25/40) of the patients by PCR. Telomerase activity as shown by TRAP-ELISA assay was detected in 82.5% (33/40) of the tumor samples and absent in 85.7% (30/35) of the normal samples taken from the same patients. As many as 53.6% (15/28) of the invasive cases were positive both for telomerase activity and for HPV, while 39.3% (11/28) of them, although indicating telomerase expression, showed no signal for HPV. This suggests that activation of telomerase could be by a pathway independent of HPV activation, although both parameters could act as diagnostic and prognostic markers for gastrointestinal tract cancers.

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References

  1. Boring CC, Squires, Tong T: Cancer Statistics, 1993. Cancer J Clin 43: 7–26, 1993

    Google Scholar 

  2. Munoz N, Day NE: Esophagus. In: D. Schottenfield, J.F. Fraumeni, Jr. (eds). Cancer Epidemiology and Prevention. Oxford University Press, New York, 1996, pp 681–706

    Google Scholar 

  3. Crespi M, Grassi A, Amiri G, Munoz N, Aramesh B, Mojtabi A, Casal: Esophageal lesions in North Iran, a premalignant condition. Lancet 2: 217, 1979

    Google Scholar 

  4. Yang CS: Research on esophageal cancer in China. A review. Cancer Res 40: 2633, 1980

    Google Scholar 

  5. Jessup JM, Menck HR, Frengen A, Winchester DP: Diagnosing colorectal carcinoma: Clinical and molecular approaches. CA Cancer J Clin 47: 70–92, 1997

    Google Scholar 

  6. Dhar GM, Shah GN, Naheed B, Hafiza: To study the distribution of cancers, with particular emphasis on esophageal cancer. J Epidemiol Community Health 47: 290–292, 1993

    Google Scholar 

  7. Zur Hausen H: Papillomaviruses in human cancers. Proc Assoc Am Physicians 111: 581–587, 1999

    Google Scholar 

  8. Lavergne D, de Villiers EM: Papillomavirus in esophageal papillomas and carcinomas. Int J Cancer 80: 681–684, 1999

    Google Scholar 

  9. Sur M, Cooper K: The role of the human papillomavirus in esophageal cancer. Pathology 30: 348–354, 1998

    Google Scholar 

  10. Anderson S, Shera K, Ihle J, Billman L, Goff B, Greer B, Tamini H, McDougall J, Klingelhutz A: Telomerase activation in cervical cancer. Am J Cancer 151: 25–31, 1997

    Google Scholar 

  11. Harley CB, Villeponteau B: Role of telomere in cancer progression. Curr Opin Genet Dev 5: 249–255, 1995

    Google Scholar 

  12. Shay JW, Werbin H, Wright WT: You haven't heard the end of it. Telomeres loss links human aging with cancer. Can J Aging 14: 511–524, 1995

    Google Scholar 

  13. Hallam N, Gibson J, Charnock M: Detection and typing of human papillomavirus infection of uterine cervix by dot blot hybridization: Comparison of scrapes and biopsies. J Med Virol 27: 317–321, 1989

    Google Scholar 

  14. Kim NW, Piatyzek MA, Prowse KR, Harley CB, West MD, Ho Peter LC, Coviello GM, Wright WE, Weinrich SL, Shay JW: Specific association of human telomerase activity with immortal cells and cancer. Science 266: 2011–2015, 1994

    Google Scholar 

  15. Cheng AJ, Tang R, Way JY, Chang JT, Wang TCV: Polymerase chain reaction-based enzyme immunoassay for quantitation of telomerase activity: Application to colorectal cancers. J Cancer Res 90: 280–285, 1999

    Google Scholar 

  16. Enterline H, Thompson J: Pathology of the Esophagous. Springer-Verlag, New York, 1985, p 145

    Google Scholar 

  17. Lagergren JM, Zhaohui W, Reinhold B, Joakim D, Nyren O: Human papillomavirus and esophageal cancer: A nationwide seroepidemiologic case-control study in Sweden. J Natl Cancer Inst 91: 156–162, 2000

    Google Scholar 

  18. Gammon MD, Schoenberg JB, Ahsan H, Risch HA, Vaughan TL, Chow WH, Rotterdam H, West AB, Dubrow R, Stanford JL, Mayre ST, Farrow DC, Niwa S, Blot WJ, Fraumeni JF: Tobacco, alcohol and socioeconomic status and adenocarcinoma of the esophagus and gastric cardia. J Natl Cancer Inst 89: 1277–1284, 1997

    Google Scholar 

  19. Weimin Ye, Ekstrom Am, Hansson LE, Bergstrom R, Nyren O: Tobacco, alcohol and the risk of gastric cancer by sub-site and histologic type. Int J Cancer 83: 223–229, 1999

    Google Scholar 

  20. Ries I, Kosary CL, Hankey B et al.: Cancer Statistics - Review, 1973-1994. NIH Publication 97: 2789, 1997

    Google Scholar 

  21. Syrjanen K: Histological changes identical to those of condylomatous lesions found in esophageal squamous cell carcinoma. Arch Geschwulstforch 52: 283–292, 1982

    Google Scholar 

  22. Das BC, Sharma JK, Gopalkrishna V, Das DK, Singh V, Gissmann L, Zur Hausen, Luthra UK: A high frequency of human papillomavirus DNA sequences in cervical carcinoma of Indian women as revealed by Southern blot hybridization and PCR. J Med Virol 36: 239–245, 1992

    Google Scholar 

  23. Sobti RC, Capalash NC, Sehgal S, Gupta BD, Sharma P: Incidence of human papillomavirus in patients with invasive cervical cancer. Cancer Genet Cytogenet 88: 175–180, 1996

    Google Scholar 

  24. Williamson AL, Dennis SJ: The use of polymerase chain reaction for the detection of human papillomavirus type 13. J Virol Methods 31: 57–65, 1991

    Google Scholar 

  25. Chang F, Syrjanen S, Wang L, Syrjanen K: Infectious agents in the etiology of esophageal cancer. Gastroenterology 103: 1336–1348, 1992

    Google Scholar 

  26. Benamouzig R, Pigot F, Kiuroga G, Validire P, Chaussade S, Catalau F, Conturier D: Human papillomavirus infection in esophageal squamous cell-carcinoma in western countries. Int J Cancer 50: 549–532, 1992

    Google Scholar 

  27. Tsao SW, He D, Bu H: Human papillomavirus infection and esophageal squamous cell carcinoma. Chung Hua Ping Li Hsuch Tsa Chih 25: 351–354, 1996

    Google Scholar 

  28. Miller BA, Davidson M, Meyerson D, Icenogle J, Lanie AP, Tan J: Human papillomavirus type 1 DNA in esophageal carcinoma from Alaska natives. Int J Cancer 7: 218–222, 1997

    Google Scholar 

  29. Loke SL, Ma L, Wong M, Srivastava G, Lo I, Bird CL: Human papillomaviruses in esophageal squamous cell carcinomas. J Clin Pathol 43: 909–912, 1990

    Google Scholar 

  30. Benamouzig R, Jullian E, Chang F, Robaskiewicz M, Flejou J-F, Raoul J-L, Coste T, Conturier D, Pompidon A, Rautureau J: Absence of human papillomavirus DNA detected by polymerase chain reaction in French patients with esophageal carcinoma. Gastroenterology 109: 1881, 1995

    Google Scholar 

  31. Turner JR, Chin LH, Crum CP, Dhan PJ, Odze RD: The prevalence of human papillomavirus infection in esophageal squamous cell carcinoma from North America: Analysis by a highly sensitive and specific polymerase chain reaction based approach. Hum Pathol 28: 174–178, 1997

    Google Scholar 

  32. De Villiers EM, Lavergne D, Chang F, Syrjanen K, Tosi P, Cintorino M, Santopietro R, Syrjanen S: An interlaboratory study to determine the presence of human papillomavirus DNA in esophageal carcinoma from China. Int J Cancer 81: 225–228, 1999

    Google Scholar 

  33. Dillner J, Knekt P, Schiller JT, Hakulinen T: Prospective seroepidemiological evidence that human papillomavirus type 16 infection is a risk factor for esophageal squamous cell carcinoma. Br Med J 311: 1346, 1995

    Google Scholar 

  34. Fidalgo PO, Cravo ML, Chaves PP, Leitao CN, Mira FC: High prevalence of human papillomavirus in squamous cell carcinoma and matched normal esophageal mucosa. Cancer Statistics - Review, 1973-1994. NIH Publication 97: 2789, 1995

    Google Scholar 

  35. Shindoh M, Chiba I, Yasuda M, Saito T, Funaoka K, Kohgo T, Amesmuja J, Sanvaday, Fujinaga K: Detection of human papillomavirus DNA sequences in oral squamous cell carcinomas and their relation to p53 and proliferating cell nuclear antigen expression. Cancer 76: 1513–1521: 1995

    Google Scholar 

  36. Bosch FX, Schwarz E, Boukampo P, Fusening NE, Bartsch D, Zur Hausen H: Suppression in vivo of human papillomavirus type 18 E6-E7 gene expression in non-tumorigenic HeLa Fibroblast hybrid cells. J Virol 64: 4743–4754, 1990

    Google Scholar 

  37. Kawai K, Yaginuma Y, Tsuruoka H, Griffin M, Hayashi H, Ishikawa M: Telomerase activity and human papillomavirus (HPV) infection in human uterine cervical cancers and cervical smears. Eur J Cancer 34: 2082–2086, 1998

    Google Scholar 

  38. Nair P, Jayaprakash PG, Nair MK, Pillai MR, Pillai MR: In press, 2000

  39. Choo CK, Ling MT, Tsao SW, Zheng Z, Zhang D, Chan LC, Wong YC: Immortalization of human prostate epithelial cells by HPV 16 E6/E7 open reading frames. Prostate 40: 150–155, 1999

    Google Scholar 

  40. Stoppler H, Hartmann DP, Sherman L, Schlegel R: The human papillomavirus type 16 E6 and E7 oncoproteins dissociate cellular telomerase activity from their maintenance of telomere length. J Biol Chem 272: 13332–13337, 1997

    Google Scholar 

  41. Joysius JK, Scott A, McDougall JK: Telomerase activation by the E6 gene product of human papillomavirus type 16. Nature 380: 79–81, 1996

    Google Scholar 

  42. Autexier C, Greider CW: Telomerase and cancer. TIBS 21: 387–391, 1996

    Google Scholar 

  43. Wisman GB, Hollema H, de Jong S, ter Schegget J, Tong-A-Hung SP, Ruiters MH, Krans M, de Vries EG, Vander Zee AG: Telomerase activity as a biomarker for (pre) neoplastic cervical disease in scrapings and frozen sections from patients with abnormal cervical smear. J Clin Oncol 2238–2245, 1998

  44. Klingelhutz JJ, Foster SA, McDougall JK: Telomerase activation by the E6 gene product of human papillomavirus type 16. Nature 380: 79–81, 1996

    Google Scholar 

  45. Mutirangura A, Sriuranpong V, Termrunggraunglert W, Tresukosol D, Lertdsgusndinchai P, Voravud N, Niruthisard S: Telomerase activity and human papillomavirus in malignant, premalignant and benign cervical lesions. Br J Cancer 78: 933–939, 1998

    Google Scholar 

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Authors and Affiliations

  1. Department of Biotechnology, Panjab University, Chandigarh-, 160014, India E-mail

    R.C. Sobti, Jaspreet Kochar & Neena Capalash

  2. Department of Gastroenterology, PGIMER, Chandigarh-, 160012, India

    K. Singh & D. Bhasin

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  1. R.C. Sobti
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  2. Jaspreet Kochar
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  3. K. Singh
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  4. D. Bhasin
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Correspondence to R.C. Sobti.

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Sobti, R., Kochar, J., Singh, K. et al. Telomerase activation and incidence of HPV in human gastrointestinal tumors in North Indian population. Mol Cell Biochem 217, 51–56 (2001). https://doi.org/10.1023/A:1007224001047

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