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MUC1, The Renaissance Molecule

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Journal of Mammary Gland Biology and Neoplasia Aims and scope Submit manuscript

Abstract

MUC1 is a large, heavily glycosylated mucin expressed on the apical surfaces of most simple, secretory epithelia including the mammary gland, gastrointestinal, respiratory, urinary and reproductive tracts. Although MUC1 was thought to be an epithelial-specific protein, it is now known to be expressed on a variety of hematopoietic cells as well. Mucins function in protection and lubrication of epithelial surfaces. Transmembrane mucins, which contain cytoplasmic tail domains, appear to have additional functions through their abilities to interact with many proteins involved in signal transduction and cell adhesion. The goal of this review is to highlight recent discoveries that suggest that MUC1 may be a multifunctional protein, located on the surfaces of cells as a sensor of the environment, poised to signal to the interior when things go awry.

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REFERENCES

  1. J. Perez-Vilar and R. L. Hill (1999). The structure and assembly of secreted mucins. J. Biol. Chem. 274:31751-31754.

    PubMed  Google Scholar 

  2. S. J. Gendler, J. M. Burchell, T. Duhig, D. Lamport, R. White, M. Parker, and J. Taylor-Papadimitriou (1987). Cloning of partial cDNA encoding differentiation and tumor-associated mucin glycoproteins expressed by human mammary epithelium. Proc. Natl. Acad. Sci. U.S.A. 84:6060-6064.

    PubMed  Google Scholar 

  3. S. J. Gendler, C. A. Lancaster, J. Taylor-Papadimitriou, T. Duhig, N. Peat, J. Burchell, L. Pemberton, E.-N. Lalani, and D. Wilson (1990). Molecular cloning and expression of human tumor-associated polymorphic epithelial mucin. J. Biol. Chem. 265:15286-15293.

    PubMed  Google Scholar 

  4. C. A. Lancaster, N. Peat, T. Duhig, D. Wilson, J. Taylor-Papadimitriou, and S. J. Gendler (1990). Structure and expression of the human polymorphic epithelial mucin gene: An expressed VNTR unit. Biochem. Biophys. Res. Commun. 173:1019-1029.

    PubMed  Google Scholar 

  5. M. S. Lan, S. K. Batra, W. N. Qi, R. S. Metzgar, and M. A. Hollingsworth (1990). Cloning and sequencing of a human pancreatic tumor mucin cDNA. J. Biol. Chem. 265:15294-15299.

    PubMed  Google Scholar 

  6. M. J. Ligtenberg, H. L. Vos, A. M. Gennissen, and J. Hilkens (1990). Episialin, a carcinoma-associated mucin, is generated by a polymorphic gene encoding splice variants with alternative amino termini. J. Biol. Chem. 265:5573-5578.

    PubMed  Google Scholar 

  7. D. H. Wreschner, M. Hareuveni, I. Tsarfaty, N. Smorodinsky, J. Horev, J. Zaretsky, P. Kotkes, M. Weiss, R. Lathe, and A. Dion (1990). Human epithelial tumor antigen cDNA sequences. Differential splicing may generate multiple protein forms. Eur. J. Biochem. 189:463-473.

    PubMed  Google Scholar 

  8. A. P. Spicer, G. Parry, S. Patton, and S. J. Gendler (1991). Molecular cloning and analysis of the mouse homologue of the tumor-associated mucin, MUC1, reveals conservation of potential O-glycosylation sites, transmembrane, and cytoplasmic domains and a loss of minisatellite-like polymorphism. J. Biol. Chem. 266:15099-15109.

    PubMed  Google Scholar 

  9. H. L. Vos, Y. de Vries, and J. Hilkens (1991). The mouse episialin (Muc1) gene and its promoter: Rapid evolution of the repetitive domain in the protein. Biochem. Biophys. Res. Commun. 181:121-130.

    PubMed  Google Scholar 

  10. S. Muller, K. Alving, J. Peter-Katalinic, N. Zachara, A. A. Gooley, and F. G. Hanisch (1999). High density Oglycosylation on tandem repeat peptide from secretory MUC1 of T47D breast cancer cells. J. Biol. Chem. 274:18165-18172.

    PubMed  Google Scholar 

  11. F. G. Hanisch and S. Muller (2000). MUC1: The polymorphic appearance of a human mucin. Glycobiology 10:439-449.

    PubMed  Google Scholar 

  12. M. E. Bramwell, G. Wiseman, and D. M. Shotton (1986). Electron-microscopic studies of the CA antigen, epitectin. J. Cell Sci. 86:249-261.

    PubMed  Google Scholar 

  13. S. Zotter, P. C. Hageman, A. Lossnitzer, W. J. Mooi, and J. Hilgers (1988). Tissue and tumor distribution of human polymorphic epithelial mucin. Cancer Reviews 11/12:55-101.

    Google Scholar 

  14. M. Boshell, E.-N. Lalani, L. Pemberton, J. Burchell, S. J. Gendler, and J. Taylor-Papadimitriou (1992). The product of the human MUC1 gene when secreted by mouse cells transfected with the full-length cDNA lacks the cytoplasmic tail. Biochem. Biophys. Res. Commun. 185:1-8.

    PubMed  Google Scholar 

  15. J. Burchell, D. Wang, and J. Taylor-Papadimitriou (1984). Detection of the tumour-associated antigens recognized by the monoclonal antibodies HMFG-1 and 2 in serum from patients with breast cancer. Int. J. Cancer 34:763-768.

    PubMed  Google Scholar 

  16. K. Zhang, D. Baeckstrom, H. Brevinge, and G. C. Hansson (1996). Secreted MUC1 mucins lacking their cytoplasmic part and carrying sialyl-Lewis a and x epitopes from a tumor cell line and sera of colon carcinoma patients can inhibit HL-60 leukocyte adhesion to E-selection-expressing endothelial cells. J. Cell Biochem. 60:538-549.

    PubMed  Google Scholar 

  17. M. A. McGuckin, P. L. Devine, L. E. Ramm, and B. G. Ward (1994). Factors effecting the measurement of tumor-associated MUC1 mucins in serum. Tumour Biol. 15:33-44.

    PubMed  Google Scholar 

  18. R. S. Metzgar, N. Rodriguez, O. J. Finn, M. S. Ian, V. N. Daasch, P. D. Fernsten, W. C. Meyers, W. F. Sindelar, and R. S. Sandler (1984). Detection of a pancreatic cancer-associated antigen (DU-PAN-2 antigen) in serum and ascites of patients with adenocarcinoma. Proc. Natl. Acad. Sci. U.S.A. 81:5242-5246.

    PubMed  Google Scholar 

  19. S. P. Treon, P. Maimonis, D. Bua, G. Young, N. Raje, J. Mollick, D. Chauhan, Y. T. Tai, T. Hideshima, Y. Shima, J. Hilgers, S. von Mensdorff-Pouilly, A. R. Belch, L. M. Pilarshi, and K. C. Anderson (2000). Elevated soluble MUC1 levels and decreased anti-MUC1 antibody levels in patients with multiple myeloma. Blood 96:3147-3153.

    PubMed  Google Scholar 

  20. L. Pemberton, J. Taylor-Papadimitriou, and S. J. Gendler (1992). Antibodies to the cytoplasmic domain of the MUC1 mucin show conservation throughout mammals. Biochem. Biophys. Res. Commun. 185:167-175.

    PubMed  Google Scholar 

  21. M. J. L. Ligtenberg, L. Kruijshaar, F. Buijs, M. van Meijer, S. V. Litvinov, and J. Hilkens (1992). Cell-associated episialin is a complex containing two proteins derived from a common precursor. J. Biol. Chem. 267:6171-6177.

    PubMed  Google Scholar 

  22. S. Zrihan-Licht, A. Baruch, O. Elroy-Stein, I. Keydar, and D. H. Wreschner (1994). Tyrosine phosphorylation of the MUC1 breast cancer membrane proteins. Cytokine receptor-like molecules. FEBS Lett. 356:130-136.

    PubMed  Google Scholar 

  23. C. J. Williams, D. H. Wreschner, A. Tanaka, I. Tsarfaty, I. Keydar, and A. S. Dion (1990). Multiple protein forms of the human breast tumor-associated epithelial membrane antigen (EMA) are generated by alternative splicing and induced by hormonal stimulation. Biochem. Biophys. Res. Commun. 170:1331-1338.

    PubMed  Google Scholar 

  24. A. Baruch, M. Hartmann, M. Yoeli, Y. Adereth, S. Greenstein, Y. Stadler, Y. Skornik, J. Zaretsky, N. I. Smorodinsky, I. Keydar, and D. H. Wreschner (1999). The breast cancer-associated MUC1 gene generates both a receptor and its cognate binding protein. Cancer Res. 59:1552-1561.

    PubMed  Google Scholar 

  25. A. Baruch, M. Hartmann, S. Zrihan-Licht, S. Greenstein, M. Burstein, I. Keydar, M. Weiss, N. Smorodinsky, and D. H. Wreschner (1997). Preferential expression of novel MUC1 tumor antigen isoforms in human epithelial tumors and their tumor-potentiating function. Int. J. Cancer 71:741-749.

    PubMed  Google Scholar 

  26. M. Hartman, A. Baruch, I. Ron, Y. Aderet, M. Yoeli, O. Sagi-Assif, S. Greenstein, Y. Stadler, M. Weiss, E. Harness, M. Yaakubovits, I. Keydar, N. I. Smorodinsky, and D. H. Wreschner (1999). MUC1 isoform specific monoclonal antibody 6E6/2 detects preferential expression of the novel MUC1/Y protein in breast and ovarian cancer. Int. J. Cancer 82:256-267.

    PubMed  Google Scholar 

  27. H. M. Oosterkamp, L. Scheiner, M. C. Stefanova, K. O. Lloyd, and C. L. Finstad (1997). Comparison of MUC-1 mucin expression in epithelial and non-epithelial cancer cell lines and demonstration of a new short variant form (MUC-1/Z). Int. J. Cancer 72:87-94.

    PubMed  Google Scholar 

  28. V. M. M. Braga, L. F. Pemberton, T. Duhig, and S. J. Gendler (1992). Spatial and temporal expression of an epithelial mucin, Muc-1, during mouse development. Development 115:427-437.

    PubMed  Google Scholar 

  29. J. Hilkens, F. Buijs, J. Hilgers, P. Hageman, J. Calafat, A. Sonnenberg, and M. van derValk (1984). Monoclonal antibodies against human milk-fat globule membranes detecting differentiation antigens of the mammary gland and its tumors. Int. J. Cancer 34:197-206.

    PubMed  Google Scholar 

  30. J. Z. Zaretsky, M. Weiss, I. Tsarfaty, M. Hareuveni, D. H. Wreschner, and I. Keydar (1990). Expression of genes coding for pS2, c-erbB2, estrogen receptor and the H23 breast tumor-associated antigen. A comparative analysis in breast cancer. FEBS Lett. 265:46-50.

    PubMed  Google Scholar 

  31. A. Girling, J. Bartkova, J. Burchell, S. Gendler, C. Gillet, and J. Taylor-Papadimitriou (1989). A core protein epitope of the polymorphic epithelial mucin detected by the monoclonal antibody SM-3 is selectively exposed in a range of primary carcinomas. Int. J. Cancer 43:1072-1076.

    PubMed  Google Scholar 

  32. J. Burchell, S. Gendler, J. Taylor-Papadimitrious, A. Girling, A. Lewis, R. Millis, and D. Lamport (1987). Development and characterization of breast cancer reactive monoclonal antibodies directed against to the core protein of the human milk mucin. Cancer Res. 47:5476-5482.

    PubMed  Google Scholar 

  33. F. Guddo, A. Giatromanolaki, M. I. Koukourakis, C. Reina, A. M. Vignola, G. Chlouverakis, J. Hilkens, K. C. Gatter, A. L. Harris, and G. Bonsignore (1998). MUC1 (episialin) expression in non-small cell lung cancer is independent of EGFR and c-erbB-2 expression and correlates with poor survival in node positive patients. J. Clin. Pathol. 51:667-671.

    PubMed  Google Scholar 

  34. S. Takao, K. Uchikura, S. Yonezawa, H. Shinchi, and T. Aikou (1999). Mucin core protein expression in extrahepatic bile duct carcinoma is associated with metastases to the liver and poor prognosis. Cancer 86:1966-1975.

    PubMed  Google Scholar 

  35. S. Nakamori, D. M. Ota, K. R. Cleary, K. Shirotani, and T. Irimura (1994). MUC1 mucin expression as a marker of progression and metastasis of human colorectal carcinoma. Gastroenterology 106:353-361.

    PubMed  Google Scholar 

  36. M. A. McGuckin, M. D. Walsh, B. G. Hohn, B. G. Ward, and R. G. Wright (1995). Prognostic significance of MUC1 epithelial mucin expression in breast cancer. Hum. Pathol. 26:432-439.

    PubMed  Google Scholar 

  37. T. Utsunomiya, S. Yonezawa, H. Sakamoto, H. Kitamura, S. Hokita, T. Aiko, S. Tanaka, T. Irimura, Y. S. Kim, and E. Sato (1998). Expression of MUC1 and MUC2 mucins in gastric carcinomas: Its relationship with the prognosis of the patients. Clin. Cancer Res. 4:2605-2614.

    PubMed  Google Scholar 

  38. Y. Dong, M. D. Walsh, M. C. Cummings, R. G. Wright, S. K. Khoo, P. G. Parsons, and M. A. McGuckin (1997). Expression of MUC1 and MUC2 mucins in epithelial ovarian tumours. J. Pathol. 183:311-317.

    PubMed  Google Scholar 

  39. H. Kitamura, S. Yonezawa, S. Tanaka, Y. S. Kim, and E. Sato (1996). Expression of mucin carbohydrates and core proteins in carcinomas of the ampulla of Vater: Their relationship to prognosis. Jpn. J. Cancer Res. 87:631-640.

    PubMed  Google Scholar 

  40. M. Sagara, S. Yonezawa, K. Nagata, Y. Tezuka, S. Natsugoe, P. X. Xing, I. F. McKenzie, T. Aikou, and E. Sato (1999). Expression of mucin 1 (MUC1) in esophageal squamous-cell carcinoma: Its relationship with prognosis. Int. J. Cancer 84:251-257.

    PubMed  Google Scholar 

  41. R. T. Greenlee, T. Murray, S. Bolden, and P. A. Wingo (2000). Cancer statistics, 2000. CA Cancer J. 50:7-33.

    Google Scholar 

  42. B. Agrawal, M. J. Krantz, J. Parker, and B. M. Longenecker (1998). Expression of MUC1 mucin on activated human T Cells—Implications for a role of MUC1 in normal immune regulation. Cancer Res. 58:4079-4081.

    PubMed  Google Scholar 

  43. S. P. Treon, J. A. Mollick, M. Urashima, G. Teoh, D. Chauhan, A. Ogata, N. Raje, J. H. M. Hilgers, L. Nadler, A. R. Belch, L. M. Pilarski, and K. C. Anderson (1999). MUC1 core protein is expressed on multiple myeloma cells and is induced by dexamethasone. Blood 93:1287-1298.

    PubMed  Google Scholar 

  44. G. A. Dent, C. J. Civalier, M. E. Brecher, and S. A. Bentley (1999). MUC1 expression in hematopoietic tissues. Am. J. Clin. Pathol. 111:741-747.

    PubMed  Google Scholar 

  45. W. Brugger, H. J. Buhring, F. Grunebach, W. Vogel, S. Kaul, R. Muller, T. H. Brummendorf, B. L. Ziegler, I. Rappold, P. Brossart, S. Scheding, and L. Kanz (1999). Expression of MUC-1 epitopes on normal bone marrow: Implications for the detection of micrometastatic tumor cells. J. Clin. Oncol. 17:1535-1544.

    PubMed  Google Scholar 

  46. T. Takahashi, Y. Makiguchi, Y. Hinoda, H. Kakiuchi, N. Nakagawa, K. Imai, and A. Yachi (1994). Expression of MUC1 on myeloma cells and induction of HLA-unrestricted CTL against MUC1 from a multiple myeloma patient. J. Immunol. 153:2102-2109.

    PubMed  Google Scholar 

  47. P. Mukherjee, A. R. Ginardi, and S. J. Gendler (2001). Defective lymphocyte function in mice lacking Muc1. Manuscript submitted.

  48. I. C. Gaemers, H. L. Vos, H. H. Volders, S. W. van DerValk, and J. Hilkens (2001). ASTAT-responsive element in the promoter of the episialin/MUC1 gene is involved in its overexpression in carcinoma cells. J. Biol. Chem. 276:6191-6199.

    PubMed  Google Scholar 

  49. A. Kovarik, N. Peat, D. Wilson, S. J. Gendler, and J. Taylor-Papadimitriou (1993). Analysis of the tissue specific promoter of the MUC1 gene. J. Biol. Chem. 268:9917-9926.

    PubMed  Google Scholar 

  50. A. Kovarik, P. J. Lu, N. Peat, J. Morris, and J. Taylor-Papadimitriou (1996). Two GC boxes (Sp1 sites) are involved in regulation of the activity of the epithelium-specific MUC1 promoter. J. Biol. Chem. 271:18140-18147.

    PubMed  Google Scholar 

  51. I. Bieche and R. Lidereau (1997). A gene dosage effect is responsible for high overexpression of the MUC1 gene observed in human breast tumors. Cancer Genet. Cytogenet. 98:75-80.

    PubMed  Google Scholar 

  52. V. G. Dyomin, N. Palanisamy, K. O. Lloyd, K. Dyomina, S. C. Jhanwar, J. Houldsworth, and R. S. Chaganti (2000). MUC1 is activated in a B-cell lymphoma by the t(1;14)(q21;q32) translocation and is rearranged and amplified in B-cell lymphoma subsets. Blood 95:2666-2671.

    PubMed  Google Scholar 

  53. F. Gilles, A. Goy, Y. Remache, P. Shue, and A. D. Zelenetz (2000). MUC1 dysregulation as the consequence of a t(1;14)(q21;q32) translocation in an extranodal lymphoma. Blood 95:2930-2936.

    PubMed  Google Scholar 

  54. S. Clark, M. A. McGuckin, T. Hurst, and B. G. Ward (1994). Effect of interferon-gamma and TNF-alpha on MUC1 mucin expression in ovarian carcinoma cell lines. Dis Markers 12:43-50.

    PubMed  Google Scholar 

  55. G. Parry, J. C. Beck, L. Moss, J. Bartley, and G. K. Ojakian (1990). Determination of apical membrane polarity in mammary epithelial cell cultures: The role of cell-cell, cellsubstratum, and membrane-cytoskeleton interactions. Exp. Cell Res. 188:302-311.

    PubMed  Google Scholar 

  56. K. Kondo, N. Kohno, A. Yokoyama, and K. Hiwada (1998). Decreased MUC1 expression induces E-cadherin-medicated cell adhesion of breast cancer cell lines. Cancer Res. 58:2014-2019.

    PubMed  Google Scholar 

  57. N. A. Hey, R. A. Graham, M. W. Seif, and J. D. Aplin (1994). The polymorphic epithelial mucin MUC1 in human endometrium is regulated with maximal expression in the implantation phase. J. Clin. Endocrinol. Metab. 78:337-342.

    PubMed  Google Scholar 

  58. M. Abe and D. Kufe (1993). Characterization of cis-acting elements regulating transcription of the human DF3 breast carcinoma-associated antigen (MUC1) gene. Proc. Natl. Acad. Sci. U.S.A. 90:282-286.

    PubMed  Google Scholar 

  59. X. Zhou, M. M. DeSouza, J. Julian, S. J. Gendler, and D. D. Carson (1998). Estrogen receptor does not directly regulate the murine Muc-1 promoter. Mol. Cell Endocrinol. 143:65-78.

    PubMed  Google Scholar 

  60. K. Shirotani and T. Irimura (1998). Purification of nuclear proteins that potentially regulate transcription of the MUC1 mucin gene induced by a soluble factor. J. Biochem. (Tokyo) 124:585-590.

    Google Scholar 

  61. M. A. Hollingsworth, C. Closken, A. Harris, C. D. McDonald, G. S. Pahwa, and L. J. Maher, 3rd (1994). A nuclear factor that binds purine-rich, single-stranded oligonucleotides derived from S1-sensitive elements upstream of the CFTR gene and the MUC1 gene. Nucleic Acids Res. 22:1138-1146.

    PubMed  Google Scholar 

  62. G. A. Surveyor, S. J. Gendler, L. Pemberton, S. K. Das, I. Chakraborty, J. Julian, R. A. Pimental, C. C. Wegner, S. K. Dey, and D. D. Carson (1995). Expression and steroid hormonal control of Muc-1 in the mouse uterus. Endocrinology 136:3639-3647.

    PubMed  Google Scholar 

  63. V. M. M. Braga and S. J. Gendler (1993). Modulation of Muc-1 mucin expression in the mouse uterus during estrus cycle, early pregnancy and placentation. J. Cell. Sci. 105:397-405.

    PubMed  Google Scholar 

  64. J. Z. Zaretsky, R. Sarid, Y. Aylon, L. A. Mittelman, D. H. Wreschner, and I. Keydar (1999). Analysis of the promoter of the MUC1 gene overexpressed in breast cancer. FEBS Lett. 461:189-195.

    PubMed  Google Scholar 

  65. D. Baeckstrom, G. C. Hansson, O. Nilsson, C. Johansson, S. J. Gendler, and L. Lindholm (1991). Purification and characterization of a membrane-bound and a secreted mucin-type glycoprotein carrying the carcinoma-associated sialyl-Lewis a epitope on distinct core proteins. J. Biol. Chem. 266:21537-21547.

    PubMed  Google Scholar 

  66. I. Brockhausen, J. M. Yang, J. Burchell, C. Whitehouse, and J. Taylor-Papadimitriou (1995). Mechanisms underlying aberrant glycosylation of MUC1 mucin in breast cancer cells. Eur. J. Biochem. 233:607-617.

    PubMed  Google Scholar 

  67. J. Burchell, R. Poulsom, A. Hanby, C. Whitehouse, L. Cooper, H. Clausen, D. Miles, and J. Taylor-Papadimitriou (1999). An alpha2,3 sialyltransferase (ST3Gal I) is elevated in primary breast carcinomas. Glycobiology 9:1307-1311.

    PubMed  Google Scholar 

  68. F.-G. Hanisch, G. Uhlenbruck, J. Peter-Katalinic, H. Egge, U. Dabrowski, and J. Dabrowski (1989). Unbranched polylactosamino-O-glycans on human skim milk mucins exhibit Gal beta (1-4) GlcNAc beta (1-6) repeating units. Symp. Soc. Exp. Biol. 43:155-162.

    PubMed  Google Scholar 

  69. J. Burchell and J. Taylor-Papadimitriou (1993). Effect of modification of carbohydrate side chains on the reactivity of antibodies with core-protein epitopes of the MUC1 gene product. Epithelial Cell Biol. 2:155-162.

    PubMed  Google Scholar 

  70. K. O. Lloyd, J. Burchell, V. Kudryashov, B. W. T. Yin, and J. Taylor-Papadimitriou (1996). Comparison of O-linked carbohydrate chains in MUC-1 mucin from normal breast epithelial cell lines and breast carcinoma cell lines. Demonstration of simpler and fewer glycan chains in tumor cells. J. Biol. Chem. 271:33325-33334.

    PubMed  Google Scholar 

  71. L. Perey, D. F. Hayes, and D. Kufe (1992). Effects of differentiating agents on cell surface expression of the breast carcinoma-associated DF3-P epitope. Cancer Res. 52:6365-6370.

    PubMed  Google Scholar 

  72. M. R. Price, P. D. Rye, E. Petrakou, A. Murray, K. Brady, S. Imai, S. Haga, Y. Kiyozuka, D. Schol, M. F. Meulenbroek, F. G. Snijdewint, S. von Mensdorff-Pouilly, R. A. Verstraeten, P. Kenemans, A. Blockzjil, K. Nilsson, O. Nilsson, M. Reddish, M. R. Suresh, R. R. Koganty, S. Fortier, L. Baronic, A. Berg, M. B. Longenecker, and J. Hilgers (1998). Summary report on the ISOBM TD-4 Workshop: Analysis of 56 monoclonal antibodies against the MUC1 mucin. San Diego, California, November 17–23, 1996. Tumour Biol. 19:1-20.

    Google Scholar 

  73. R. R. Parmley and S. J. Gendler (1998). Cystic fibrosis mice lacking Muc1 have reduced amounts of intestinal mucus. J. Clin. Invest. 102:1798-1806.

    PubMed  Google Scholar 

  74. M. Hinojosa-Kurtzberg, G. C. Hansson, and S. J. Gendler (2000). A high molecular weight MUC1 mucin is overexpressed in the intestinal lumen of CF mice. Pediatr. Pulmonol. Suppl. 20:220.

    Google Scholar 

  75. R. Kardon, R. E. Price, J. Julian, E. Lagow, S. C. Tseng, S. J. Gendler, and D. D. Carson (1999). Bacterial conjunctivitis in Muc1 null mice. Invest Ophthalmol. Vis. Sci. 40:1328-1335.

    PubMed  Google Scholar 

  76. M. M. DeSouza, G. A. Surveyor, R. E. Price, J. Julian, R. Kardon, X. Zhou, S. Gendler, J. Hilkens, and D. D. Carson (1999). MUC1/episialin: A critical barrier in the female reproductive tract. J. Reprod. Immunol. 45:127-158.

    PubMed  Google Scholar 

  77. H. Schroten, F. G. Hanisch, R. Plogmann, J. Hacker, G. Uhlenbruck, R. Nobis-Bosch, and V. Wahn (1992). Inhibition of adhesion of S-fimbriated Escherichia coli to buccal epithelial cells by human milk fat globule membrane components: A novel aspect of the protective function of mucins in the nonimmunoglobulin fraction. Infect Immun. 60:2893-2899.

    PubMed  Google Scholar 

  78. R. H. Yolken, J. A. Peterson, S. L. Vonderfecht, E. T. Fouts, K. Midthun, and D. S. Newburg (1992). Human milk mucin inhibits rotavirus replication and prevents experimental gastroenteritis. J. Clin. Invest. 90:1984-1991.

    PubMed  Google Scholar 

  79. K. C. Kim, B. T. Kim, X. G. Zhang, D. I. Lee, and S. W. Hyun (2000). Pseudomonas Aeruginosa as a potential ligand for Muc1 mucins on the cell surface. In Mucins in Health and Disease, Cambridge, UK, p. 58.

  80. R. J. Quin and M. A. McGuckin (2000). Phosphorylation of the cytoplasmic domain of the MUC1 mucin correlates with changes in cell-cell adhesion. Int. J. Cancer 87:499-506.

    PubMed  Google Scholar 

  81. J. D. Fontenot, N. Tjandra, D. Bu, C. Ho, R. C. Montelaro, and O. J. Finn (1993). Biophysical characterization of one-, two-, and three-tandem repeats of human mucin (muc-1) protein core. Cancer Res. 53:5386-5394.

    PubMed  Google Scholar 

  82. N. Jentoft (1990). Why are proteins O-glycosylated? TIBS 15:291-294.

    PubMed  Google Scholar 

  83. M. J. Ligtenberg, F. Buijs, H. L. Vos, and J. Hilkens (1992). Suppression of cellular aggregation by high levels of episialin. Cancer Res. 52:2318-2324.

    PubMed  Google Scholar 

  84. J. Wesseling, S. W. van der Valk, H. L. Vos, A. Sonnenberg, and J. Hilkens (1995). Episialin (MUC1) overexpression inhibits integrin-mediated cell adhesion to extracellular matrix components. J. Cell Biol. 129:255-265.

    PubMed  Google Scholar 

  85. J. Wesseling, S. W. van der Valk, and J. Hilkens (1996). A mechanism for inhibition of E-cadherin-mediated cell-cell adhesion by the membrane-associated mucin episialin/MUC1. Mol. Biol. Cell. 7:565-577.

    PubMed  Google Scholar 

  86. M. Yamamoto, A. Bharti, Y. Li, and D. Kufe (1997). Interaction of the DF3/MUC1 breast carcinoma-associated antigen and beta-catenin in cell adhesion. J. Biol. Chem. 272:12492-12494.

    PubMed  Google Scholar 

  87. Y. Q. Li, A. Bharti, D. S. Chen, J. L. Gong, and D. Kufe (1998). Interaction of glycogen synthase kinase 3-beta with the DF3/MUC1 carcinoma-associated antigen and beta-catenin. Mol. Cell. Biol. 18:7216-7224.

    PubMed  Google Scholar 

  88. T. Suwa, Y. Hinoda, Y. Makiguchi, T. Takahashi, F. Itoh, M. Adachi, M. Hareyama, and K. Imai (1998). Increased invasiveness of MUC1 and cDNA-transfected human gastric cancer MKN74 cells. Int. J. Cancer 76:377-382.

    PubMed  Google Scholar 

  89. M. J. Hudson, G. W. Stamp, M. A. Hollingsworth, M. Pignatelli, and E. N. Lalani (1996). MUC1 expressed in PanC1 cells decreases adhesion to type 1 collagen but increases contraction in collagen lattices. Am. J. Pathol. 148:951-960.

    PubMed  Google Scholar 

  90. S. Satoh, Y. Hinoda, T. Hayashi, M. D. Burdick, K. Imai, and M. A. Hollingsworth (2000). Enhancement of metastatic properties of pancreatic cancer cells by MUC1 gene encoding an anti-adhesion molecule [In Process Citation]. Int. J. Cancer 88:507-518.

    PubMed  Google Scholar 

  91. R. Sikut, K. Zhang, D. Baeckstrom, and G. C. Hansson (1996). Distinct sub-populations of carcinoma-associated MUC1 mucins as detected by the monoclonal antibody 9H8 and antibodies against the sialyl-Lewis a and sialyl-Lewis x epitopes in the circulation of breast-cancer patients. Int. J. Cancer 66:617-623.

    PubMed  Google Scholar 

  92. C. Hanski, K. Drechsler, F. G. Hanisch, J. Sheehan, M. Manske, D. Ogorek, E. Klussmann, M. L. Hanski, M. Blank, P. X. Xing, I. F. C. McKenzie, P. L. Devine, and E. D. Riecken (1993). Altered glycosylation of the MUC-1 protein core contributes to the colon carcinoma-associated increase of mucin-bound sialyl-Lewis(x) expression. Cancer Res. 53:4082-4088.

    PubMed  Google Scholar 

  93. K. Zhang, D. Baeckstrom, H. Brevinge, and G. C. Hansson (1997). Comparison of sialyl-Lewis a-carrying CD43 and MUC1 mucins secreted from a colon carcinoma cell line for Eselectin binding and inhibition of leukocyte adhesion. Tumour Biol. 18:175-187.

    PubMed  Google Scholar 

  94. G. Mannori, P. Crottet, O. Cecconi, K. Hanasaki, A. Aruffo, R. M. Nelson, A. Varki, and M. P. Bevilacqua (1995). Differential colon cancer cell adhesion to E-, P-, and L-selectin: Role of mucin-type glycoproteins. Cancer Res. 55:4425-4431.

    PubMed  Google Scholar 

  95. K. Zhang, R. Sikut, and G. C. Hansson (1997). A MUC1 mucin secreted from a colon carcinoma cell line inhibits target cell lysis by natural killer cells. Cellular Immunology 176:158-165.

    PubMed  Google Scholar 

  96. L. H. Regimbald, L. M. Pilarski, B. M. Longenecker, M. A. Reddish, G. Zimmermann, and J. C. Hugh (1996). The breast mucin MUC1 as a novel adhesion ligand for endothelial intercellular adhesion molecule 1 in breast cancer. Cancer Res. 56:4244-4249.

    PubMed  Google Scholar 

  97. J. L. Kam, L. H. Regimbald, J. H. Hilgers, P. Hoffman, M. J. Krantz, B. M. Longenecker, and J. C. Hugh (1998). MUC1synthetic peptide inhibition of intercellular adhesion molecule-1 and MUC1 binding requires six tandem repeats. Cancer Res. 58:5577-5581.

    PubMed  Google Scholar 

  98. A. P. Spicer, T. Duhig, B. S. Chilton, and S. J. Gendler (1995). Analysis of mammalian MUC1 genes reveals potential functionally important domains. Mamm. Genome. 6:885-888.

    PubMed  Google Scholar 

  99. M. Mockensturm-Gardner and S. J. Gendler (1998). Phosphorylation of MUC1 and association with p185 upon EGF stimulation. Proceedings of the American Association for Cancer Research 39:375a.

    Google Scholar 

  100. P. Pandey, S. Kharbanda, and D. Kufe (1995). Association of the DF3/MUC1 breast cancer antigen with Grb2 and the Sos/Ras exchange protein. Cancer Res. 55:4000-4003.

    PubMed  Google Scholar 

  101. J. A. Schroeder, M. C. Thompson, M. M. Gardner, and S. J. Gendler (2001). Transgenic MUC1 interacts with EGFR and correlates with MAP kinase activation in the mouse mammary gland. J. Biol. Chem. 276:13057-13064.

    PubMed  Google Scholar 

  102. M. McGuckin, M. Tran, M. Wykes, K. MacDonald, R. Quin, D. Wreschner, and D. Hart (2000). Expression and function of MUC1 on activated T cells and dendritic cells. In Mucins in Health and Disease, Cambridge, UK, p. 66.

  103. Y. Li, H. Kuwahara, J. Ren, G. Wen, and D. Kufe (2001). The c-Src Tyrosine Kinase Regulates Signaling of the Human DF3/MUC1 Carcinoma-associated Antigen with GSK3beta and beta-Catenin. J. Biol. Chem. 276:6061-6064.

    PubMed  Google Scholar 

  104. I. Alroy and Y. Yarden (1997). The ErbB signaling network in embryogenesis and oncogenesis: Signal diversification through combinatorial ligand-receptor interactions. FEBS Lett. 410:83-86.

    PubMed  Google Scholar 

  105. G. Carpenter (2000). The EGF receptor: A nexus for trafficking and signaling. Bioessays 22:697-707.

    PubMed  Google Scholar 

  106. M. A. Olayioye, D. Graus-Porta, R. R. Beerli, J. Rohrer, B. Gay, and N. E. Hynes (1998). ErbB-1 and ErbB-2 acquire distinct signaling properties dependent upon their dimerization partner. Mol. Cell Biol. 18:5042-5051.

    PubMed  Google Scholar 

  107. M. A. Olayioye, I. Beuvink, K. Horsch, J. M. Daly, and N. E. Hynes (1999). ErbB receptor-induced activation of stat transcription factors is mediated by Src tyrosine kinases. J. Biol. Chem. 274:17209-17218.

    PubMed  Google Scholar 

  108. A. P. Spicer, G. J. Rowse, T. K. Lidner, and S. J. Gendler (1995). Delayed mammary tumor progression in Muc-1 null mice. J. Biol. Chem. 270:30093-30101.

    PubMed  Google Scholar 

  109. S. J. Gendler, P. Mukherjee, A. R. Ginardi, C. Madsen, C. Sterner, M. C. Adriance, J. Schroeder, J. Rowles, N. Canales, B. Reid, M. Thompson, and M. Hinojosa-Kurtzberg (2000). Role of MUC1 in tumor progession and immune regulation. In Mucins in Health and Disease, Cambridge, UK, p. 62.

  110. E. van de Wiel-van Kemenade, M. J. Ligtenberg, A. J. de Boer, F. Buijs, H. L. Vos, C. J. Melief, J. Hilkens, and C. G. Figdor (1993). Episialin (MUC1) inhibits cytotoxic lymphocyte-target cell interaction. J. Immunol. 151:767-776.

    Google Scholar 

  111. D. L. Barnd, M. S. Lan, R. S. Metzgar, and O. J. Finn (1989). Specific, major histocompatibility complex-unrestricted recognition of tumor-associated mucins by human cytotoxic T cells. Proc. Natl. Acad. Sci. U.S.A. 86:7159-7163.

    PubMed  Google Scholar 

  112. D. F. Hayes, D. S. Silberstein, S. W. Rodrique, and D. W. Kufe (1990). DF3 antigen, ahumanepithelial cell mucin, inhibits adhesion of eosinophils to antibody-coated targets. J. Immunol. 145:962-970.

    PubMed  Google Scholar 

  113. S. Ogata, P. J. Maimonis, and S. H. Itzkowitz (1992). Mucins bearing the cancer-associated sialosyl-Tn antigen mediate inhibition of natural killer cell cytotoxicity. Cancer Res. 52:4741-4746.

    PubMed  Google Scholar 

  114. C. G. Ioannides, B. Fisk, K. R. Jerome, T. Irimura, J. T. Wharton, and O. J. Finn (1993). Cytotoxic T cells from ovarian malignant tumors can recognize polymorphic epithelial mucin core peptides. J. Immunol. 151:3693-3703.

    PubMed  Google Scholar 

  115. K. R. Jerome, N. Domenech, and O. J. Finn (1993). Tumor-specific cytotoxic T cell clones from patients with breast and pancreatic adenocarcinoma recognize EBV-immortalized B cells transfected with polymorphic epithelial mucin complementary DNA. J. Immunol. 151:1654-1662.

    PubMed  Google Scholar 

  116. N. Domenech, R. A. Henderson, and O. J. Finn (1995). Identification of an HLA-A11-restricted epitope from the tandem repeat domain of the epithelial tumor antigen mucin. J. Immunol. 155:4766-4774.

    PubMed  Google Scholar 

  117. M. Reddish, G. D. MacLean, R. R. Koganty, J. Kan-Mitchell, V. Jones, M. S. Mitchell, and B. M. Longenecker (1998). Anti-MUC1 class I restricted CTLs in metastatic breast cancer patients immunized with a synthetic MUC1 peptide. Int. J. Cancer 76:817-823.

    PubMed  Google Scholar 

  118. B. Agrawal, M. J. Krantz, M. A. Reddish, and B. M. Longenecker (1998). Rapid induction of primary human CD4+ and CD8+ T cell responses against cancer-associated MUC1 peptide epitopes. Int. Immunol. 10:1907-1916.

    PubMed  Google Scholar 

  119. K. R. Jerome, D. L. Barnd, K. M. Bendt, C. M. Boyer, J. Taylor-Papadimitriou, I. F. McKenzie, R. C. Bast, Jr., and O. J. Finn (1991). Cytotoxic T-lymphocytes derived from patients with breast adenocarcinoma recognize an epitope present on the protein core of a mucin molecule preferentially expressed by malignant cells. Cancer Res. 51:2908-2916.

    PubMed  Google Scholar 

  120. K. R. Jerome, D. Bu, and O. J. Finn (1992). Expression of tumor-associated epitopes on Epstein-Barr virus-immortalized B-cells and Burkitt's lymphomas transfected with epithelial mucin complementary DNA. Cancer Res. 52:5985-5990.

    PubMed  Google Scholar 

  121. B. Agrawal, S. J. Gendler, and B. M. Longenecker (1998). The Biological Role of Mucins in Cellular Interactions and Immune Regulation—Prospects for Cancer Immunotherapy [Review]. Molecular Medicine Today 4:397-403.

    PubMed  Google Scholar 

  122. A. K. Chan, D. C. Lockhart, W. von Bernstorff, R. A. Spanjaard, H. G. Joo, T. J. Eberlein, and P. S. Goedegebuure (1999). Soluble MUC1 secreted by human epithelial cancer cells mediates immune suppression by blocking T-cell activation. Int. J. Cancer 82:721-726.

    PubMed  Google Scholar 

  123. B. Agrawal, M. A. Reddish, M. J. Krantz, and B. M. Longenecker (1995). Does pregnancy immunize against breast cancer? Cancer Res. 55:2257-2261.

    PubMed  Google Scholar 

  124. P. L. Devine, J. Y. Yarker, K. M. Fong, M. A. McGuckin, B. Scells, B. G. Ward, G. S. Thynne, and P. V. Zimmerman (1994). Serum markers CASA, CEA, CYFRA 21-1, MSA, NSE, TPA and TPS in lung cancer. International Journal of Oncology 4:1129-1134.

    Google Scholar 

  125. A. Rughetti, V. Turchi, C. A. Ghetti, G. Scambia, P. B. Panici, G. Roncucci, S. Mancuso, L. Frati, and M. Nuti (1993). Human B-cell immune response to the polymorphic epithelial mucin. Cancer Res. 53:2457-2459.

    PubMed  Google Scholar 

  126. Y. Hinoda, N. Nakagawa, H. Nakamura, Y. Makiguchi, F. Itoh, M. Adachi, T. Yabana, K. Imai, and A. Yachi (1993). Detection of a circulating antibody against a peptide epitope on a mucin core protein, MUC1, in ulcerative colitis. Immunology Letters 35:163-168.

    PubMed  Google Scholar 

  127. Y. Kotera, J. D. Fontenot, G. Pecher, R. S. Metzgar, and O. J. Finn (1994). Humoral immunity against a tandem repeat epitope of human mucin MUC-1 in sera from breast, pancreatic, and colon cancer patients. Cancer Res. 54:2856-2860.

    PubMed  Google Scholar 

  128. E. R. Richards, P. L. Devine, R. J. Quin, J. D. Fontenot, B. G. Ward, and M. A. McGuckin (1998). Antibodies reactive with the protein core ofMUC1mucin are present in ovarian cancer patients and healthy women. Cancer Immunol. Immunother. 46:245-252.

    PubMed  Google Scholar 

  129. S. von Mensdorff-Pouilly, M. M. Gourevitch, P. Kenemans, A. A. Verstraeten, G. J. van Kamp, A. Kok, K. van Uffelen, F. G. Snijdewint, M. A. Paul, S. Meijer, and J. Hilgers (1998). An enzyme-linked immunosorbent assay for the measurement of circulating antibodies to polymorphic epithelial mucin (MUC1). Tumour Biol. 19:186-195.

    PubMed  Google Scholar 

  130. M. M. Gourevitch, S. von Mensdorff-Pouilly, S. V. Litvinov, P. Kenemans, G. J. van Kamp, A. A. Verstraeten, and J. Hilgers (1995). Polymorphic epithelial mucin (MUC-1)-containing circulating immune complexes in carcinoma patients. Br. J. Cancer 72:934-938.

    PubMed  Google Scholar 

  131. S. von Mensdorff-Pouilly, M. M. Gourevitch, P. Kenemans, A. A. Verstraeten, S. V. Litvinov, G. J. van Kamp, S. Meijer, J. Vermorken, and J. Hilgers (1996). Humoral immune response to polymorphic epithelial mucin (MUC-1) in patients with benign and malignant breast tumours. Eur. J. Cancer 32A:1325-1331.

    Google Scholar 

  132. S. von Mensdorff-Pouilly, A. A. Verstraeten, P. Kenemans, F. G. Snijdewint, A. Kok, G. J. Van Kamp, M. A. Paul, P. J. Van Diest, S. Meijer, and J. Hilgers (2000). Survival in early breast cancer patients is favorably influenced by a natural humoral immune response to polymorphic epithelial mucin. J. Clin. Oncol. 18:574-583.

    PubMed  Google Scholar 

  133. S. von Mensdorff-Pouilly, E. Petrakou, P. Kenemans, K. van Uffelen, A. A. Verstraeten, F. G. Snijdewint, G. J. van Kamp, D. J. Schol, C. A. Reis, M. R. Price, P. O. Livingston, and J. Hilgers (2000). Reactivity of natural and induced human antibodies to MUC1 mucin with MUC1 peptides and n-acetylgalactosamine (GalNAc) peptides. Int. J. Cancer 86:702-712.

    PubMed  Google Scholar 

  134. R. B. Acres, M. Hareuveni, J. M. Balloul, and M. P. Kieny (1993). Vaccinia virus MUC1 immunization of mice: Immune response and protection against the growth of murine tumors bearing the MUC1 antigen. J. Immunother. 14:136-143.

    PubMed  Google Scholar 

  135. V. Apostolopoulos, P. X. Xing, and I. F. McKenzie (1994). Murine immune response to cells transfected with human MUC1: Immunization with cellular and synthetic antigens. Cancer Res. 54:5186-5193.

    PubMed  Google Scholar 

  136. L. Ding, E.-N. Lalani, R. M., R. Koganty, T. Wong, J. Samuel, M. B. Vacyshyn, A. Meikle, P. Y. S. Fung, J. Taylor-Papadimitriou, and B. M. Longenecker (1993). Immunogenicity of synthetic peptides related to the core peptide sequence encoded by the human MUC1 mucin gene: Effect of immunization on the growth of murine mammary adenocarcinoma cells transfected with the human MUC1 gene. Cancer Immunol. Immunother. 36:9-17.

    Google Scholar 

  137. S. Zhang, L. A. Graeber, F. Helling, G. Ragupathi, S. Adluri, K. O. Lloyd, and P. O. Livingston (1996). Augmenting the immunogenicity of synthetic MUC1 peptide vaccines in mice. Cancer Res. 56:3315-3319.

    PubMed  Google Scholar 

  138. J. Samuel, W. A. Budzynski, M. A. Reddish, L. Ding, G. L. Zimmermann, M. J. Krantz, R. R. Koganty, and B. M. Longenecker (1998). Immunogenicity and antitumor activity of a liposomal MUC1 peptide-based vaccine. Int. J. Cancer 75:295-302.

    PubMed  Google Scholar 

  139. G. J. Rowse, R. M. Tempero, M. L. VanLith, M. A. Hollingsworth, and S. J. Gendler (1998). Tolerance and immunity to MUC1 in a human MUC1 transgenic murine model. Cancer Res. 58:315-321.

    PubMed  Google Scholar 

  140. B. Acres, V. Apostolopoulos, J. M. Balloul, D. Wreschner, P. X. Xing, D. Ali-Hadji, N. Bizouarne, M. P. Kieny, and I. F. McKenzie (2000). MUC1-specific immune responses in human MUC1 transgenic mice immunized with various human MUC1 vaccines. Cancer Immunol. Immunother. 48:588-594.

    PubMed  Google Scholar 

  141. N. Peat, S. J. Gendler, E.-N. Lalani, T. Duhig, and J. Taylor-Papadimitriou (1992). Tissue-specific expression of a human polymorphic epithelial mucin (MUC 1) in transgenic mice. Cancer Res. 52:1954-1960.

    PubMed  Google Scholar 

  142. P. Mukherjee, A. R. Ginardi, C. S. Madsen, C. J. Sterner, M. C. Adriance, M. J. Tevethia, and S. J. Gendler (2000). Mice with spontaneous pancreatic cancer naturally develop MUC1-specific CTLs that eradicate tumors when adoptively transferred. J. Immunol. 165:3451-3460.

    PubMed  Google Scholar 

  143. V. Apostolopoulos, G. A. Pietersz, P. X. Xing, C. J. Lees, M. Michael, J. Bishop, and I. F. McKenzie (1995). The immunogenicity of MUC1 peptides and fusion protein. Cancer Lett. 90:21-26.

    PubMed  Google Scholar 

  144. S. M. Barratt-Boyes, A. Vlad, and O. J. Finn (1999). Immunization of chimpanzees with tumor antigen MUC1 mucin tandem repeat peptide elicits both helper and cytotoxic T-cell responses. Clin. Cancer Res. 5:1918-1924.

    PubMed  Google Scholar 

  145. H. A. Vaughan, D. W. Ho, V. A. Karanikas, C. S. Ong, L. A. Hwang, J. M. Pearson, I. F. McKenzie, and G. A. Pietersz (1999). Induction of humoral and cellular responses in cynomolgus monkeys immunised with mannan-humanMUC1 conjugates. Vaccine 17:2740-2752.

    PubMed  Google Scholar 

  146. J. S. Goydos, E. Elder, T. L. Whiteside, O. J. Finn, and M. T. Lotze (1996). Aphase I trial of a synthetic mucin peptide vaccine. Induction of specific immune reactivity in patients with adenocarcinoma. Journal of Surgical Research 63:298-304.

    PubMed  Google Scholar 

  147. V. Karanikas, L. A. Hwang, J. Pearson, C. S. Ong, V. Apostolopoulos, H. Vaughan, P. X. Xing, G. Jamieson, G. Pietersz, B. Tait, R. Broadbent, G. Thynne, and I. F. C. McKenzie (1997). Antibody and T cell responses of patients with adenocarcinoma immunized with mannan-Muc1 fusion protein. J. Clin. Invest. 100:2783-2792.

    PubMed  Google Scholar 

  148. S. M. Scholl, J. M. Balloul, G. Le Goc, N. Bizouarne, C. Schatz, M. P. Kieny, S. von Mensdorff-Pouilly, A. Vincent-Salomon, L. Deneux, E. Tartour, W. Fridman, P. Pouillart, and B. Acres (2000). Recombinant vaccinia virus encoding human MUC1 and IL2 as immunotherapy in patients with breast cancer [In Process Citation]. J. Immunother. 23:570-580.

    PubMed  Google Scholar 

  149. T. Gilewski, S. Adluri, G. Ragupathi, S. Zhang, T. J. Yao, K. Panageas, M. Moynahan, A. Houghton, L. Norton, and P. O. Livingston (2000). Vaccination of high-risk breast cancer patients with mucin-1 (MUC1) keyhole limpet hemocyanin conjugate plus QS-21. Clin. Cancer Res. 6:1693-1701.

    PubMed  Google Scholar 

  150. G. A. Pietersz, W. Li, C. Osinski, V. Apostolopoulos, and I. F. McKenzie (2000). Definition of MHC-restricted CTL epitopes from non-variable number of tandem repeat sequence of MUC1. Vaccine 18:2059-2071.

    PubMed  Google Scholar 

  151. V. Apostolopoulos, G. Chelvanayagam, P. X. Xing, and I. F. McKenzie (1998). Anti-MUC1 antibodies react directly with MUC1 peptides presented by class I H2 and HLA molecules. J. Immunol. 161:767-775.

    PubMed  Google Scholar 

  152. Y. Guo, M. Wu, H. Chen, X. Wang, G. Liu, G. Li, J. Ma, and M. S. Sy (1994). Effective tumor vaccine generated by fusion of hepatoma cells with activated B cells. Science 263:518-520.

    PubMed  Google Scholar 

  153. G. Stuhler and P. Walden (1994). Recruitment of helper T cells for induction of tumour rejection by cytolytic T lymphocytes. Cancer Immunol. Immunother. 39:342-345.

    PubMed  Google Scholar 

  154. J. Gong, D. Chen, M. Kashiwaba, and D. Kufe (1997). Induction of antitumor activity by immunization with fusions of dendritic and carcinoma cells. Nat. Med. 3:558-561.

    PubMed  Google Scholar 

  155. J. Wang, S. Saffold, X. Cao, J. Krauss, and W. Chen (1998). Eliciting T cell immunity against poorly immunogenic tumors by immunization with dendritic cell-tumor fusion vaccines. J. Immunol. 161:5516-5524.

    PubMed  Google Scholar 

  156. J. Gong, D. Chen, M. Kashiwaba, Y. Li, L. Chen, H. Takeuchi, H. Qu, G. J. Rowse, S. J. Gendler, and D. Kufe (1998). Reversal of tolerance to human MUC1 antigen in MUC1 transgenic mice immunized with fusions of dendritic and carcinoma cells. Proc. Natl. Acad. Sci. U.S.A. 95:6279-6283.

    PubMed  Google Scholar 

  157. A. Kugler, G. Stuhler, P. Walden, G. Zoller, A. Zobywalski, P. Brossart, U. Trefzer, S. Ullrich, C. A. Muller, V. Becker, A. J. Gross, B. Hemmerlein, L. Kanz, G. A. Muller, and R. H. Ringert (2000). Regression of human metastatic renal cell carcinoma after vaccination with tumor cell-dendritic cell hybrids. Nat. Med. 6:332-336.

    PubMed  Google Scholar 

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    Sandra J. Gendler

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Gendler, S.J. MUC1, The Renaissance Molecule. J Mammary Gland Biol Neoplasia 6, 339–353 (2001). https://doi.org/10.1023/A:1011379725811

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