Original ContributionsMIB-1 (Ki-67), p53, estrogen receptor, and progesterone receptor expression in uterine smooth muscle tumors*,**
Section snippets
Materials and methods
Fifteen cases of cellular leiomyomata, 7 cases of smooth muscle tumor of uncertain malignant potential (STUMP), and 12 cases of leiomyosarcoma were retrieved from the files of the division of gynecologic pathology at New York University Medical Center. The diagnosis of leiomyosarcoma and STUMP was based on previously published criteria.2 Tumors with marked nuclear atypia were classified as leiomyosarcoma if they had ≥5 mitoses/10 high-power fields (HPF) and as STUMP if they had 1 to 4
Results
Results are shown in Table 1 and Fig 1 through 4. NOTE. Results are presented as means ± SE (range).Empty Cell MIB-1 p53 ER PR Cellular leiomyata 0.9 ± 0.3 (0-5) 0.1 ± 0.09 (0-1) 40 ± 8.8 (0-90) 52 ± 8.2 (0-90) STUMP 5.7 ± 1.3 (0-10) 0.1 ± 0.1 (0-1) 15 ± 9 (0-50) 84 ± 7.6 (40-95) Leiomyosarcoma 45.4 ± 8.6 (10-70)* 21.9 ± 9 (0-90)* 4 ± 4 (0-50) 5 ± 4.9 (0-60)* *P <.00001 v STUMP and cellular leiomyata.
Discussion
We chose to measure immunostaining subjectively because this method is fast and does not require any equipment other than a microscope. This method can be used not only in academic settings, but also in private practice settings. Our results are in agreement with previous studies that have used counting of cells to measure the percentage of cells positive for p53, ER, PR, and MIB-1 in leiomyosarcomas. Percentage staining, rather than number of cells stained/10 HPF, was used because it provides
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Cited by (0)
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Supported in part by Kaplan Cancer Center grant P30 CA16087.
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Address correspondence and reprint requests to Khush Mittal, MD, Department of Pathology, 4W35B, Bellevue Hospital, 27th St and 1st Ave, New York, NY 10016.