Regular ArticleValue of lysozyme agar incorporation and alkaline thioglycollate exposure for the environmental recovery ofClostridium difficile
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Approaches to the detection of Clostridioides difficile in the healthcare environment
2019, Journal of Hospital InfectionCitation Excerpt :Some authors examined plates for growth after 48 h [19,23,25,30,33] while others incubated plates for 48 h, reviewed the plates and re-incubated them for a further 72 h if culture-negative before deciding if there was no growth [26,29,32]. The duration of incubation of the enrichment broth when enrichment was used was 48 h [22,33]. Both the European Society of Clinical Microbiology and Infectious Diseases' (2014) and the Infectious Diseases Society of America's guidelines (2018) on the management of CDI support the use of PCR (and other nucleic acid amplification techniques) or an enzyme immunoassay for the detection of glutamate dehydrogenase (GDH) as the first step of a multi-step algorithm for the laboratory diagnosis of CDI [38,39].
Clostridium difficile infection in pregnant and postpartum women in 2 hospitals and a review of literature
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2014, AnaerobeCitation Excerpt :However, semi-quantification of positive broths showed that CCMB-TAL gave an improved recovery rate over CCFB (Table 1). This observation seems to confirm previous studies finding that the presence of lysozyme increases the recovery rate of C. difficile [14]. Subsequently, lysozyme was added to CCFB to see if its addition improved recovery in this medium.
Evaluation of the illumigene C. difficile assay for toxigenic Clostridium difficile detection: A prospective study of 302 consecutive clinical fecal samples
2014, Diagnostic Microbiology and Infectious Disease
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Corresponding author: Dr Mark H. Wilcox, Department of Microbiology, University of Leeds, Leeds, LS2 9JT.