Automated Mass Spectrometry: A Revolutionary Technology for Clinical Diagnostics
References (0)
Cited by (48)
Techniques for the detection, identification, and diagnosis of agricultural pathogens and diseases
2019, Natural Remedies for Pest, Disease and Weed ControlClinical Mass Spectrometry in the Bioinformatics Era: A Hitchhiker's Guide
2018, Computational and Structural Biotechnology JournalCitation Excerpt :Strategic planning of an clinical MS-based assay will not only avoid these pitfalls, but should also allow the assay to surpass the performance of traditional immunoassays due to the simultaneous discrimination and quantification of the clinically relevant sequence variations [300]. Automation of mass spectrometric analysis is still in its preliminary stage [301], and while sample preparation techniques like online solid phase extraction are available [302], the majority of clinical laboratories still employ significant amount of manual techniques in sample preparation. Henceforth round-the-clock mass spectrometry services, though reported in the literature [303], remained a rarity among clinical laboratories, at least in the authors’ experience.
Genetic mutation screen in early non-small-cell lung cancer (NSCLC) specimens
2014, Clinical Lung CancerCitation Excerpt :The extension reaction included sequence-specific hybridization and sequence-dependent termination that generated different products for the mutated and wild type alleles, each with its unique mass. Polymerase chain reaction (PCR) products were identified using mass spectrometry.16,17 Multiplexed PCR was performed 384-well plates, 5-μL volumes containing 0.1 units of Taq polymerase (Hotstar Taq, Quiagen), 50 ng of genomic DNA, 200 nmol of each PCR primer, and 0.2 mmol of deoxynucleotide (dNTP).
Interactions among genes influencing bacterial recognition increase IBD risk in a population-based New Zealand cohort
2009, Human ImmunologyCitation Excerpt :Two genotyping platforms were used to assay the different variants. The p.Lys216Glu SNP in the BPI gene (rs4358188) was genotyped with both the ABI TaqMan MGB diallelic discrimination system and the Sequenom MassArray system [44]. The promoter variants in the CD14 gene (c.-159 T>C, rs7524551) and the TLR9 gene (c.-1237 C>T, rs5743836) were genotyped with the ABI TaqMan MGB diallelic discrimination system.
Single nucleotide polymorphisms in human Paneth cell defensin A5 may confer susceptibility to inflammatory bowel disease in a New Zealand Caucasian population
2008, Digestive and Liver DiseaseCitation Excerpt :The four genotyped SNPs and their build 35 coordinates are SNP A (rs7017866; 6889 kb), SNP B (rs12682030; 6891 kb), SNP C (rs10095331; 6901 kb), and SNP D (rs4610776; 6902 kb). Genotyping was performed using the Sequenom® MassArray platform [24]. Primers, sequences and conditions are available on request.
The enhancement of homogenous mass extension reaction: Comparison of two enzymes
2007, Molecular and Cellular Probes