Neoplastic cells and proliferating endothelial cells express connective tissue growth factor (CTGF) in glioblastoma

Li-Hua Pan; Takaaki Beppu; Akira Kurose; Kohei Yamauchi; Atsushi Sugawara; Michiyasu Suzuki; Akira Ogawa; Takashi Sawai

doi:10.1179/016164102101200573

Neoplastic cells and proliferating endothelial cells express connective tissue growth factor (CTGF) in glioblastoma

Neurol Res. 2002 Oct;24(7):677-83. doi: 10.1179/016164102101200573.

Authors

Li-Hua Pan¹, Takaaki Beppu, Akira Kurose, Kohei Yamauchi, Atsushi Sugawara, Michiyasu Suzuki, Akira Ogawa, Takashi Sawai

Affiliation

¹ First Department of Pathology, Iwate Medical University School of Medicine, Morioka, Japan.

PMID: 12392205
DOI: 10.1179/016164102101200573

Abstract

Connective tissue growth factor (CTGF) was recently described as a cysteine-rich mitogenic peptide that is the prototypic member of the CCN family of protein including CTGF/fisp12, cef/10/cyr61 and nov. Recently CTGF was reported to have a function of mediating endothelial cell adhesion and migration through integrin alpha(v)beta3, promoting endothelial cell survival, and inducing angiogenesis in vivo. To clarify involvement of CTGF in tumor angiogenesis, using brain tissues with glioblastoma, we examined CTGF expression and localization by immunohistochemical staining and reverse transcription-polymerase chain reaction. The result showed that both glioblastoma tumor cells and proliferating endothelial cells are positive for CTGF by immunohistochemical staining, and CTGF mRNA expression was evaluated by RT-PCR in the brain tissues of glioblastoma. These findings suggest that CTGF may have the potential to stimulate angiogenesis in glioblastoma and function as an angiogenic factor in the process of tumor growth.

MeSH terms

Adolescent
Adult
Aged
Brain Neoplasms / blood supply
Brain Neoplasms / metabolism*
Brain Neoplasms / pathology
Cell Division / physiology*
Child
Child, Preschool
Connective Tissue Growth Factor
Endothelium, Vascular / metabolism*
Endothelium, Vascular / pathology
Female
Glial Fibrillary Acidic Protein / metabolism
Glioblastoma / blood supply
Glioblastoma / metabolism*
Glioblastoma / pathology
Humans
Immediate-Early Proteins / genetics
Immediate-Early Proteins / metabolism*
Immunohistochemistry
Infant
Intercellular Signaling Peptides and Proteins / genetics
Intercellular Signaling Peptides and Proteins / metabolism*
Male
Middle Aged
Neovascularization, Pathologic / metabolism*
Neovascularization, Pathologic / physiopathology
Nephroblastoma Overexpressed Protein
RNA, Messenger / metabolism
Up-Regulation / physiology

Substances

CCN2 protein, human
CCN3 protein, human
Glial Fibrillary Acidic Protein
Immediate-Early Proteins
Intercellular Signaling Peptides and Proteins
Nephroblastoma Overexpressed Protein
RNA, Messenger
Connective Tissue Growth Factor