The expression of minichromosome maintenance protein-2 in normal and abnormal megakaryocytes and comparison with the proliferative marker Ki-67

Br J Haematol. 2005 Nov;131(4):490-4. doi: 10.1111/j.1365-2141.2005.05801.x.

Abstract

The minichromosome maintenance (Mcm) and Cdc6 proteins are important regulators of eucaryotic DNA replication. In most normal tissues, a similar proportion of cells express Mcm-2 and Ki-67. The present study showed that in both normal and abnormal states, the proportion of megakaryocytes expressing Mcm-2 is roughly seven times as many as those that express Ki-67. This is likely to be related to the process of endomitosis and endoreduplication. We also demonstrated that a significantly lower proportion of megakaryocytes in myelodysplastic syndrome express Mcm-2. These findings provide new insights into megakaryocyte biology.

Publication types

  • Comparative Study

MeSH terms

  • Bone Marrow / metabolism
  • Cell Cycle Proteins / metabolism*
  • Cell Nucleus / metabolism
  • Cytoplasm / metabolism
  • Erythroid Precursor Cells / metabolism
  • Humans
  • Integrin beta3 / metabolism
  • Ki-67 Antigen / metabolism*
  • Megakaryocytes / metabolism*
  • Minichromosome Maintenance Complex Component 2
  • Myelodysplastic Syndromes / metabolism*
  • Myelodysplastic Syndromes / pathology
  • Myeloproliferative Disorders / metabolism*
  • Myeloproliferative Disorders / pathology
  • Nuclear Proteins / metabolism*

Substances

  • Cell Cycle Proteins
  • ITGB3 protein, human
  • Integrin beta3
  • Ki-67 Antigen
  • Nuclear Proteins
  • MCM2 protein, human
  • Minichromosome Maintenance Complex Component 2