Absence of human herpesvirus-8 and Epstein-Barr virus in inflammatory myofibroblastic tumor with anaplastic large cell lymphoma kinase fusion gene

Hidetaka Yamamoto; Kenichi Kohashi; Yoshinao Oda; Sadafumi Tamiya; Yukiko Takahashi; Yoshiaki Kinoshita; Shin Ishizawa; Masayuki Kubota; Masazumi Tsuneyoshi

doi:10.1111/j.1440-1827.2006.02012.x

Absence of human herpesvirus-8 and Epstein-Barr virus in inflammatory myofibroblastic tumor with anaplastic large cell lymphoma kinase fusion gene

Pathol Int. 2006 Oct;56(10):584-90. doi: 10.1111/j.1440-1827.2006.02012.x.

Authors

Hidetaka Yamamoto¹, Kenichi Kohashi, Yoshinao Oda, Sadafumi Tamiya, Yukiko Takahashi, Yoshiaki Kinoshita, Shin Ishizawa, Masayuki Kubota, Masazumi Tsuneyoshi

Affiliation

¹ Department of Anatomic Pathology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. hidetaka@surgpath.med.kyushu-u.ac.jp

PMID: 16984614
DOI: 10.1111/j.1440-1827.2006.02012.x

Abstract

Inflammatory myofibroblastic tumor (IMT) is clinically and histologically characterized by inflammation. Some populations of IMT have anaplastic large cell lymphoma kinase (ALK) gene rearrangements. Infection with Epstein-Barr virus (EBV) and human herpesvirus-8 (HHV-8) in tumor cells of IMT has been reported; these reports, however, have been limited to ALK-negative IMT. The purpose of the present paper was to evaluate 21 cases of IMT for the presence of EBV and HHV-8. Immunohistochemically, 15 cases were ALK positive and six were negative. Of eight cases analyzed using reverse transcription-polymerase chain reaction, tropomyosin 3 (TPM3)-ALK, TPM4-ALK and clathrin heavy chain-ALK fusion genes were detected in one, two and two cases, respectively. All 21 IMT, irrespective of ALK expression, were negative for EBV by in situ hybridization for EBV-encoded RNA and immunohistochemical stain for latent membrane antigen-1. HHV-8 was also negative in all IMT by PCR for HHV-8 DNA sequence (KS330/233) and immunohistochemical stain for latent nuclear antigen. These results suggest that IMT may be a heterogeneous group in terms of pathogenesis, and EBV and HHV-8 do not play a major role in the pathogenesis of ALK-positive tumor.

Publication types

Evaluation Study

MeSH terms

Adolescent
Adult
Aged
Anaplastic Lymphoma Kinase
Antigens, Viral / genetics
Antigens, Viral / metabolism
Child
Child, Preschool
Clathrin Heavy Chains / genetics
Clathrin Heavy Chains / metabolism
Epstein-Barr Virus Infections / complications*
Epstein-Barr Virus Infections / genetics
Epstein-Barr Virus Infections / pathology
Female
Gene Expression Regulation, Neoplastic / genetics
Gene Expression Regulation, Viral
Herpesviridae Infections / complications*
Herpesviridae Infections / genetics
Herpesviridae Infections / pathology
Herpesvirus 4, Human / genetics
Herpesvirus 4, Human / pathogenicity*
Herpesvirus 8, Human / genetics
Herpesvirus 8, Human / pathogenicity*
Humans
Infant
Male
Middle Aged
Neoplasms, Muscle Tissue / etiology
Neoplasms, Muscle Tissue / genetics*
Neoplasms, Muscle Tissue / pathology
Neoplasms, Muscle Tissue / virology*
Nuclear Proteins / genetics
Nuclear Proteins / metabolism
Oncogene Proteins, Fusion / genetics
Protein-Tyrosine Kinases / genetics*
Protein-Tyrosine Kinases / metabolism
Receptor Protein-Tyrosine Kinases
Tropomyosin / genetics
Tropomyosin / metabolism

Substances

Antigens, Viral
Nuclear Proteins
Oncogene Proteins, Fusion
TPM3 protein, human
TPM4 protein, human
Tropomyosin
latency-associated nuclear antigen
Clathrin Heavy Chains
ALK protein, human
Anaplastic Lymphoma Kinase
Protein-Tyrosine Kinases
Receptor Protein-Tyrosine Kinases