Abstract
The effects of a novel polypeptide, pancreatic spasmolytic polypeptide (PSP) on a colon carcinoma cell line (HCT 116) were examined. PSP stimulated the incorporation of [3H]thymidine into HCT 116 cells as well as cell proliferation in a dose-dependent manner. Maximal increase in [3H]thymidine incorporation of 50-60% occurred at 3-300 microM PSP. The VIP-mediated-increase in cAMP levels was reduced by PSP at greater than 1 microM concentrations. PSP is highly homologous to the estrogen-induced pS2 protein in MCF-7 breast cancer cells. We find that PSP also enhanced [3H]thymidine incorporation in MCF-7 cells. These findings indicate for the first time that PSP has growth stimulatory properties.
MeSH terms
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Animals
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Breast Neoplasms / metabolism
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Breast Neoplasms / pathology*
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Cell Division / drug effects
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Colonic Neoplasms / metabolism
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Colonic Neoplasms / pathology*
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Cyclic AMP / metabolism
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DNA / biosynthesis
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Humans
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Intercellular Signaling Peptides and Proteins
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Mucins*
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Muscle Proteins*
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Neuropeptides*
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Parasympatholytics
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Peptides / pharmacology*
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Swine
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Trefoil Factor-2
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Trefoil Factor-3
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Tumor Cells, Cultured
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Vasoactive Intestinal Peptide / metabolism
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Vasoactive Intestinal Peptide / pharmacology
Substances
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Intercellular Signaling Peptides and Proteins
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Mucins
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Muscle Proteins
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Neuropeptides
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Parasympatholytics
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Peptides
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TFF3 protein, rat
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Tff2 protein, rat
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Trefoil Factor-2
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Trefoil Factor-3
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Vasoactive Intestinal Peptide
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pancreatic spasmolytic polypeptide
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DNA
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Cyclic AMP