Abstract
Prenatal typing for the human platelet antigens-1 (HPA) permits identification of a fetus at risk for neonatal alloimmune thrombocytopenia (NAITP) in cases of HPA-1 incompatibility in which the father is heterozygous for the HPA-1a antigen. Diagnostic cordocentesis and phenotyping of the fetal platelets are used for this purpose. We applied allele-specific restriction enzyme analysis on polymerase chain reaction (PCR)-amplified DNA purified from amniocytes. This assays allows early second trimester typing for HPA-1 alleles. We were able to determine the genotype of three fetuses at risk. Iatrogenic fetal loss is lower with amniocentesis than with cordocentesis. Therefore, this technique is a welcome addition to the antenatal management of NAITP.
MeSH terms
-
Alleles
-
Amniocentesis
-
Amniotic Fluid / cytology*
-
Antigens, Human Platelet / genetics
-
Antigens, Human Platelet / immunology*
-
Blood Grouping and Crossmatching*
-
Cells, Cultured
-
Cordocentesis
-
DNA / genetics*
-
DNA / isolation & purification
-
Deoxyribonuclease HpaII
-
Deoxyribonucleases, Type II Site-Specific
-
Female
-
Fetal Blood / chemistry*
-
Humans
-
Immunization
-
Integrin beta3
-
Isoantibodies / blood*
-
Male
-
Pedigree
-
Polymerase Chain Reaction*
-
Polymorphism, Restriction Fragment Length
-
Pregnancy
-
Prenatal Diagnosis*
-
Risk
-
Safety
-
Thrombocytopenia / genetics
-
Thrombocytopenia / immunology
-
Thrombocytopenia / prevention & control*
Substances
-
Antigens, Human Platelet
-
ITGB3 protein, human
-
Integrin beta3
-
Isoantibodies
-
DNA
-
Deoxyribonuclease HpaII
-
Deoxyribonucleases, Type II Site-Specific