Human platelet antigen-1 (Zw) typing of fetuses by analysis of polymerase chain reaction-amplified genomic DNA from amniocytes

Transfus Med. 1994 Mar;4(1):15-9. doi: 10.1111/j.1365-3148.1994.tb00238.x.

Abstract

Prenatal typing for the human platelet antigens-1 (HPA) permits identification of a fetus at risk for neonatal alloimmune thrombocytopenia (NAITP) in cases of HPA-1 incompatibility in which the father is heterozygous for the HPA-1a antigen. Diagnostic cordocentesis and phenotyping of the fetal platelets are used for this purpose. We applied allele-specific restriction enzyme analysis on polymerase chain reaction (PCR)-amplified DNA purified from amniocytes. This assays allows early second trimester typing for HPA-1 alleles. We were able to determine the genotype of three fetuses at risk. Iatrogenic fetal loss is lower with amniocentesis than with cordocentesis. Therefore, this technique is a welcome addition to the antenatal management of NAITP.

Publication types

  • Case Reports

MeSH terms

  • Alleles
  • Amniocentesis
  • Amniotic Fluid / cytology*
  • Antigens, Human Platelet / genetics
  • Antigens, Human Platelet / immunology*
  • Blood Grouping and Crossmatching*
  • Cells, Cultured
  • Cordocentesis
  • DNA / genetics*
  • DNA / isolation & purification
  • Deoxyribonuclease HpaII
  • Deoxyribonucleases, Type II Site-Specific
  • Female
  • Fetal Blood / chemistry*
  • Humans
  • Immunization
  • Integrin beta3
  • Isoantibodies / blood*
  • Male
  • Pedigree
  • Polymerase Chain Reaction*
  • Polymorphism, Restriction Fragment Length
  • Pregnancy
  • Prenatal Diagnosis*
  • Risk
  • Safety
  • Thrombocytopenia / genetics
  • Thrombocytopenia / immunology
  • Thrombocytopenia / prevention & control*

Substances

  • Antigens, Human Platelet
  • ITGB3 protein, human
  • Integrin beta3
  • Isoantibodies
  • DNA
  • Deoxyribonuclease HpaII
  • Deoxyribonucleases, Type II Site-Specific