Abstract
We established and characterized a new human synovial sarcoma cell line, SN-SY-1, derived from a monophasic fibrous synovial sarcoma excised from the abdominal wall of a 21-year-old man. We maintained the cell line for over 35 passages in vitro. The SN-SY-1 cells in vitro exhibit spindle and polygonal shapes and join to form an epithelial plaque. SN-SY-1 maintained a consistent karyotype: 46,t(X;18)(p11.2;q11.2), Y, t(6;8) (q13-15;q11-13), the same as that of the primary tumor specimen. RT-PCR assay for chimeric SYT-SSX transcripts followed by digestion with restriction enzymes revealed SYT-SSX1 fusion protein. An immunohistochemical study revealed expression of bcl-2 protein in the SN-SY-1 cell line, in vitro and in vivo. However, DNA analysis showed no rearrangement of the bcl-2 gene, and expression of bcl-2 mRNA was low. Why bcl-2 protein was expressed in this synovial sarcoma was not clarified.
MeSH terms
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Abdominal Neoplasms / genetics
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Abdominal Neoplasms / metabolism
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Abdominal Neoplasms / pathology
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Abdominal Neoplasms / surgery
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Adult
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Cell Culture Techniques / methods
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Cell Division
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Cell Line
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Child
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Chromosome Mapping
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Chromosomes, Human, Pair 18
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Female
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Genes, bcl-2
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Humans
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Karyotyping
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Male
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Middle Aged
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Neoplasm Proteins*
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Polymerase Chain Reaction
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Protein Biosynthesis*
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Protein Multimerization
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Proteins*
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-bcl-2 / biosynthesis*
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RNA, Messenger / biosynthesis
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Recombinant Fusion Proteins / biosynthesis
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Repressor Proteins / biosynthesis*
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Sarcoma, Synovial / genetics
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Sarcoma, Synovial / metabolism
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Sarcoma, Synovial / pathology*
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Sarcoma, Synovial / surgery
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Transcription, Genetic*
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Translocation, Genetic
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Tumor Cells, Cultured
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X Chromosome
Substances
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Neoplasm Proteins
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Proteins
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-bcl-2
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RNA, Messenger
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Recombinant Fusion Proteins
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Repressor Proteins
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SS18 protein, human
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synovial sarcoma X breakpoint proteins