Abstract
Expression of gene transcripts for immunoglobulins and a T-cell receptor was investigated in non-hematopoietic tumor cell lines using the highly sensitive RT-nested PCR method. These proteins are reported to be produced and secreted or expressed in malignancies originating from hematopoietic organs only. Originally designed PCR primers for different exons coding for the heavy-chain constant regions of IgM, IgD, IgG3, IgG1, IgE, and IgA and the T-cell receptor-alpha were used. All gene transcripts were detected in the 5 investigated cancer cell lines without exception. The results suggest that even non-hematopoietic cancer cells transcribe immunoglobulin and T-cell receptor genes and may produce the corresponding proteins.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenocarcinoma / genetics*
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Adenocarcinoma / immunology*
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Animals
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Breast Neoplasms / genetics
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Breast Neoplasms / immunology
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Carcinoma, Squamous Cell / genetics
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Carcinoma, Squamous Cell / immunology
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Chickens
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Colonic Neoplasms / genetics
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Colonic Neoplasms / immunology
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Gene Expression / immunology*
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Genes, Immunoglobulin*
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Genes, T-Cell Receptor / genetics*
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Humans
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Immunoglobulin Constant Regions / biosynthesis
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Immunoglobulin Constant Regions / genetics*
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Immunoglobulin Heavy Chains / biosynthesis
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Immunoglobulin Heavy Chains / genetics*
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Laryngeal Neoplasms / genetics
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Laryngeal Neoplasms / immunology
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Oryzias
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Transcription, Genetic / immunology*
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Tumor Cells, Cultured
Substances
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Immunoglobulin Constant Regions
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Immunoglobulin Heavy Chains