Abstract

A rapid technique for the determination of iron in serum, after the intravenous injection of iron-dextran, is presented.

Dithionite is used for the reduction of ferric to ferrous iron.

The use of a detergent solution enables protein precipitation to be omitted.

The iron reagent, 4: 7-diphenyl-1: 10-phenanthroline, and its ferrous complex are both soluble in the dilute detergent solution.

Lipaemic serum can be used without affecting the results.

The recovery of added ferric iron, as iron-dextran, is quantitative.