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The determination of urinary 17-ketosteroids by an improved Zimmermann reaction
  1. R. N. Beale,
  2. J. O. Bostrom,
  3. D. Croft
  1. Institute of Clinical Pathology and Medical Research, Department of Public Health, Sydney, Australia 1


    Improvements in the Zimmermann reaction are effected by using methanol as a solvent for the reagents employed in the reaction in place of ethanol or aqueous ethanol. The advantages are as follows. Considerably lower blanks are obtained; extraneous chromogens are negligible at the wavelength of measurement, so that no correction procedure or extraction process is required; high concentrations of potassium hydroxide may be used at an elevated temperature leading to good sensitivity and a considerably shortened reaction time; and finally, the potassium hydroxide is stable for long periods under good storage conditions. A steroid with keto (oxo) groups at positions 3, 11, and 20, but not at 17, failed to give any coloured product.

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    • 1 Postal address: Institute of Clinical Pathology and Medical Research, Post Office Box 108, Lidcombe, New South Wales, Australia.