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The estimation of uranin (fluorescien sodium) in blood
  1. T. Wagatsuma,
  2. Helen Payling Wright
  1. Institute of Obstetrics and Gynaecology, Hammersmith Hospital, London


    The extraction and estimation of uranin (fluorescein sodium) in plasma is described. Heparin-plasma containing uranin is digested with crude ficin and extracted with acid n-pentanol. The supernatant fluid is re-extracted with N NaOH and, after volume adjustment, read against a uranin standard in a fluorimeter with appropriate filters. It appears reliable within 4% at concentrations as low as 10−8 g./ml.

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