Article Text

Download PDFPDF
Rapid microbiological assay of serum vitamin B12 by electronic counter
  1. J. Stuart,
  2. S. A. Sklaroff
  1. Department of Haematology, Royal Infirmary, Edinburgh
  2. Department of Public Health and Social Medicine, University of Edinburgh


    A new method of measuring the growth of Lactobacillus leichmannii is reported. Its adoption for the estimation of serum vitamin B12 levels shortens the incubation period required to five hours at 45°C. The method is compared statistically with a standard method of estimation, requiring incubation at 37°C., by duplicate determinations on 106 hospital patients. The significance of the apparently decreased accuracy of the new method at low serum levels is discussed, and a re-appraisal of the optimum growth temperature of Lactobacillus leichmannii suggested.

    Statistics from

    Request Permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.