Article Text

Download PDFPDF
An automated method for serum bilirubin determination
  1. N. A. Simmons1
  1. Department of Clinical Pathology, Guy's Hospital, London


    A method for the determination of both direct and indirect serum bilirubin on the AutoAnalyzer is described which has a number of advantages over the automated method that is currently recommended. It gives total bilirubin results which are similar to those obtained with the equivalent manual method and direct bilirubin results which are usually about 10% lower.

    The effect of haemolysis on estimations was investigated. It has little influence on the total, but significantly lowers the direct bilirubin results.

    Simple methods for the preparation of true bilirubin standards and a stable control serum are described.

    Statistics from

    Request Permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.


    • 1 Present address: Department of Pathology, Chase Farm Hospital, Enfield, Middlesex.