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Use of peroxidase-conjugated antiglobulin as an alternative to immunofluorescence for the detection of antinuclear factor in serum
  1. J. Dorling,
  2. G. D. Johnson,
  3. Janet A. Webb,
  4. Mavis E. Smith
  1. Medical Research Council Rheumatism Unit, Canadian Red Cross Memorial Hospital, Taplow, Maidenhead, Berkshire


    A method of detecting serum antinuclear factor by the use of antiglobulin conjugated with horseradish peroxidase has been compared with a standard procedure using a fluorescent label. The horseradish peroxidase conjugate was evaluated by block titration against positive serum and by its performance as a test reagent for screening and titrating sera. It is concluded that the horse-radish peroxidase method, in which an ordinary light microscope is employed, provides a satisfactory alternative to immunofluorescence for routine tests for antinuclear factor.

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