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Observations on interactions between fluorescein-iso-thiocyanate conjugates and cells in vitro
  1. G. Sander1,
  2. J. P. Jacobs
  1. National Institute for Medical Research (Hampstead Laboratories), London


    During the course of some routine immunofluorescence studies `non-specific' staining occurred in the absence of free fluorescein and of any known impurities. The cause of such staining seems to be associated with phasic changes occurring at varying population doublings (`passages') in species-homologous cells subjected to serial cultivation.

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    • 1 Present address: The Mathilda & Terence Kennedy Institute of Rheumatology, Bute Gardens, Hammersmith, London, W6 7DW