Article Text

Download PDFPDF
The separation of epinephrine from norepinephrine and dopamine from DOPA on Sephadex G-10
  1. T. J. McDonald,
  2. B. E. P. Murphy
  1. Endocrinology Laboratory, Queen Mary Veterans Hospital, Montreal, Canada
  2. Department of Experimental Medicine, McGill University, Montreal, Canada

    Abstract

    Epinephrine and norepinephrine were separated by acid elution through a Sephadex G-10 column with high recovery (better than 90%), excellent reproducibility, and little overlap (less than 10%). Once packed, the columns could be re-used indefinitely. Total elution time was about six hours and the columns could be left untended since the gravity flow stops automatically once the level of the eluant reaches the gel bed. The resulting dilution was five-fold. 3,4-Dihydroxyphenylalanine (DOPA) was completely separated from 3,4-dihydroxyphenylethylamine (dopamine) by the same procedure.

    Statistics from Altmetric.com

    Request Permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.