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Selective media in the isolation of tubercle bacilli from tissues
  1. D. A. Mitchison,
  2. B. W. Allen,
  3. R. A. Lambert
  1. Department of Bacteriology, Royal Postgraduate Medical School, London


    Direct culture on Lowenstein-Jensen slopes and on three media made selective for tubercle bacilli by the addition of four antibacterial agents was compared with guinea-pig inoculation on 490 tissue specimens. Tubercle bacilli were obtained from 15 specimens by culture and 14 by guinea-pig inoculation; only one specimen was positive by guinea-pig and not by culture. The most efficient culture medium was a selective 7H11 slope. Routine guinea-pig inoculation has been replaced by a wider range of culture procedures.

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