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An automated passive haemagglutination technique suitable for the detection of hepatitis B virus antigen and antibody in blood donors
  1. Margaret Nelson1,
  2. Elizabeth J. Phipps,
  3. Pamela G. Watson,
  4. Jacqueline R. Watts,
  5. Roman Zwolenski
  1. NSW Red Cross Blood Transfusion Service and Technicon Equipment, Sydney, Australia

    Abstract

    A method is described for preparing purified hepatitis B antigen (HB Ag) viral antigen without density gradient centrifugation. A method for sensitizing human group O red cells with this preparation is given, together with the technical details of an automated passive haemagglutination technique suitable for the mass screening of blood donors for HB virus and anti-HB antibody.

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    Footnotes

    • 1 Address for reprints: Mrs Margaret Nelson, Biochemistry Department, NSW Red Cross Blood Transfusion Service, 1 York Street, Sydney NSW 2000, Australia