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Plasma alkaline phosphatase isoenzymes in hepatobiliary disease
  1. A. O. Afonja1,
  2. D. N. Baron2
  1. Department of Chemical Pathology, Royal Free Hospital, London


    By cellulose acetate or acrylamide gel electrophoresis it is possible to separate these alkaline phosphatase isoenzymes from serum: [anode] fast liver, slow liver, placenta/Regan, bone, intestine, bile [cathode]. Heat or chemical inhibition can confirm the differentiation.

    Normal adult serum always contains slow-liver isoenzyme, and sometimes bone isoenzyme: the latter is always present in serum of children. In hepatobiliary disease slow-liver isoenzyme was always increased: intestinal isoenzyme appeared in many cases of cirrhosis (of blood groups B and 0) but fast-liver and bile isoenzymes were occasionally seen in miscellaneous cases. The findings in other diseases included Regan isoenzyme in six out of 45 cases of malignant disease.

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    • 1 Present address: Department of Chemical Pathology, Lagos University Teaching Hospital, Nigeria.

    • 2 Correspondence to Professor D. N. Baron, Department of Chemical Pathology, Royal Free Hospital, London NW3 2QG.